Iushkova E A, Zaĭnullin V G
Genetika. 2014 May;50(5):515-21.
This paper studies the effect of chronic γ-radiation of different intensities on the induction of hobo-elements in cells ofdysgenetic and non-dysgenetic drosophila species. The level of gonadal atrophy, DNA damage, and mutability of the mini-white locus is estimated. It is shown that the frequency of displacements of the hobo-elements increases with the increase in the chronic irradiation dose, where an essential role belongs to the maternal effect. The level of DNA damage in the cells of embryos and larvae varies and depends on the conditions of induction of hobo-transposons. Analysis of the PCR products showed that chronic irradiation in a certain range of accumulated doses is able to induce formation of new copies of the hobo-elements. At the same time, the structure of deleted hobo-sequences may vary in response to higher doses of irradiation.
本文研究了不同强度的慢性γ辐射对发育异常和发育正常的果蝇物种细胞中hobo元件诱导的影响。评估了性腺萎缩水平、DNA损伤以及mini-white基因座的突变性。结果表明，hobo元件的位移频率随着慢性辐射剂量的增加而增加，其中母体效应起着重要作用。胚胎和幼虫细胞中的DNA损伤水平各不相同，并且取决于hobo转座子的诱导条件。对PCR产物的分析表明，在一定累积剂量范围内的慢性辐射能够诱导hobo元件新拷贝的形成。与此同时，缺失的hobo序列结构可能会因更高剂量的辐射而发生变化。
