Chubukova O V, Baĭmiev An Kh, Mudarisova A F, Baĭmiev Al Kh, Muldashev A A
Genetika. 2014 May;50(5):531-7.
The carbohydrate-binding sequences (CBS) of the lectin genes from legume plants of the genera Astragalus Lam., Oxytropis DC., and Hedysarum L. were determined. Computer-assisted analysis of nucleotide and predicted amino acid sequences of the lectin gene fragments examined revealed a high homology level between their CBS. At the same time, the CBS of Astragalus and Oxytropis were considerably different from the lectin gene CBS in the earlier examined representatives of the tribe Galegeae, Caragana frutex and C. arborescens. This fact probably points to the differences in the carbohydrate-binding specificity of the proteins examined, which can eventually affect their functional activity.
测定了黄芪属(Astragalus Lam.)、棘豆属(Oxytropis DC.)和岩黄芪属(Hedysarum L.)豆科植物凝集素基因的碳水化合物结合序列(CBS)。对所检测的凝集素基因片段的核苷酸和预测氨基酸序列进行计算机辅助分析,结果显示它们的CBS之间具有高度同源性。同时,黄芪属和棘豆属的CBS与 earlier examined representatives of the tribe Galegeae(原文此处表述有误,推测可能是指锦鸡儿族的早期研究代表植物)的凝集素基因CBS有很大差异,如锦鸡儿(Caragana frutex)和树锦鸡儿(C. arborescens)。这一事实可能表明所检测蛋白质的碳水化合物结合特异性存在差异,最终可能会影响它们的功能活性。