Kaledin V I, Il'nitskaia S I, Ovchinnikova L P, Popova N A, Bogdanova L A, Morozkova T S
Biofizika. 2014 May-Jun;59(3):527-32.
It is found that after administration of 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB,) which was hepatocarcinogenic to rats, in suckling mice, the number of neoplastic lesions in the liver of mice was 3 times higher than after analogous administration of equimolar dose of ortho-aminoazotoluene (OAT)). However, in the Ames test (TA-98 strain of Salmonella typhimurium) with activation by hepatic enzymes (S-9 fraction) of both intact and Aroclor-1254-induced mice and rats OAT contributed by an order of magnitude to revertant colonies compared to 3'-Me-DAB. In vivo inhibition of sulfotransferase activity, the enzyme which catalyzes the final stage of the mutagenic activation of aminoazo dyes, had no effect on carcinogenicity of 3'-Me-DAB but more than 4 times elevated that of OAT. It was concluded that the mechanism of carcinogenic action of aminoazo dyes studied is not genotoxic and that the carcinogenic potential of OAT is lost in the process of mutagenic activation.
研究发现，给哺乳期小鼠注射对大鼠具有致癌性的3'-甲基-4-二甲基氨基偶氮苯（3'-Me-DAB）后，小鼠肝脏中的肿瘤性病变数量比注射等摩尔剂量的邻氨基偶氮甲苯（OAT）后高出3倍。然而，在使用完整小鼠和经多氯联苯混合物Aroclor-1254诱导的小鼠及大鼠的肝酶（S-9组分）进行活化的艾姆斯试验（鼠伤寒沙门氏菌TA-98菌株）中，与3'-Me-DAB相比，OAT产生的回复突变菌落数量要高出一个数量级。体内抑制硫酸转移酶活性（该酶催化氨基偶氮染料诱变活化的最后阶段）对3'-Me-DAB的致癌性没有影响，但使OAT的致癌性提高了4倍多。得出的结论是，所研究的氨基偶氮染料的致癌作用机制不是遗传毒性的，并且OAT的致癌潜力在诱变活化过程中丧失。
