Mo Chunling, Yang Yueyue, Chen Ning, Yang Xiushan, Tian Shen
Sheng Wu Gong Cheng Xue Bao. 2014 Sep;30(9):1401-13.
In this study, we constructed a yeast consortium surface-display expression system by using Flo1 as an anchor protein. Endoglucanase II (EGII) and cellobiohydrolase II (CBHII) from Trichoderma reesei, and β3-glucosidase 1 (BGLI) from Aspergillus aculeatus were immobilized on Saccharomyces cerevisiae Y5. We constructed the cellulose-displaying expression yeast consortium (Y5/fEGII:Y5/fCBHII:Y5/fBGLI = 1:1:1) and investigated the enzymatic ability and ethanol fermentation. The displayed cellulolytic enzymes was stabile during the 96-h fermentation. The yeast consortium produced 0.77 g/L ethanol from 10 g/L phosphoric acid swollen cellulose (PASC) within 96 h. The yield (in grams of ethanol produced per gram of carbohydrate consumed) was 0.35 g/g, which correspond to 68.6% of the theoretical yield.
在本研究中，我们以Flo1作为锚定蛋白构建了一个酵母联合体表面展示表达系统。将里氏木霉的内切葡聚糖酶II（EGII）和纤维二糖水解酶II（CBHII）以及棘孢曲霉的β3-葡萄糖苷酶1（BGLI）固定在酿酒酵母Y5上。我们构建了展示纤维素的表达酵母联合体（Y5/fEGII:Y5/fCBHII:Y5/fBGLI = 1:1:1），并研究了其酶活性和乙醇发酵情况。展示的纤维素分解酶在96小时发酵过程中保持稳定。该酵母联合体在96小时内从10 g/L磷酸膨胀纤维素（PASC）中产生了0.77 g/L乙醇。产率（每消耗一克碳水化合物产生的乙醇克数）为0.35 g/g，相当于理论产率的68.6%。
