在高度分化的人呼吸道上皮细胞培养物上对人冠状病毒的特性研究。
Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures.
作者信息
Jonsdottir Hulda R, Dijkman Ronald
机构信息
Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, 3012, Switzerland.
出版信息
Methods Mol Biol. 2015;1282:73-87. doi: 10.1007/978-1-4939-2438-7_8.
Abstract
The human airway serves as the entry point of human respiratory viruses, including human coronaviruses. In this chapter we outline the methods by which we establish fully differentiated airway epithelium and its use for human coronavirus propagation. Additionally, we outline methods for immunofluorescence staining of these cultures for virus detection, characterization of cell tropism, and how to perform antiviral assays and quantify viral replication.
摘要
人类气道是包括人类冠状病毒在内的人类呼吸道病毒的进入点。在本章中，我们概述了建立完全分化的气道上皮的方法及其在人类冠状病毒繁殖中的应用。此外，我们还概述了对这些培养物进行免疫荧光染色以检测病毒、表征细胞嗜性的方法，以及如何进行抗病毒测定和量化病毒复制。
相似文献
1
Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures.在高度分化的人呼吸道上皮细胞培养物上对人冠状病毒的特性研究。
Methods Mol Biol. 2015;1282:73-87. doi: 10.1007/978-1-4939-2438-7_8.
2
Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures.高分化原代哺乳动物气道上皮细胞培养物
Methods Mol Biol. 2020;2203:119-134. doi: 10.1007/978-1-0716-0900-2_10.
3
The Cell Tropism of Porcine Respiratory Coronavirus for Airway Epithelial Cells Is Determined by the Expression of Porcine Aminopeptidase N.猪呼吸冠状病毒对气道上皮细胞的细胞嗜性由猪氨基肽酶 N 的表达决定。
Viruses. 2020 Oct 23;12(11):1211. doi: 10.3390/v12111211.
4
Tropism and Infectivity of a Seasonal A(H1N1) and a Highly Pathogenic Avian A(H5N1) Influenza Virus in Primary Differentiated Ferret Nasal Epithelial Cell Cultures.季节性 A(H1N1)和高致病性禽流感 A(H5N1)流感病毒在原代分化雪貂鼻上皮细胞培养物中的趋向性和感染性。
J Virol. 2019 May 1;93(10). doi: 10.1128/JVI.00080-19. Print 2019 May 15.
5
Culturing the unculturable: human coronavirus HKU1 infects, replicates, and produces progeny virions in human ciliated airway epithelial cell cultures.培养无法培养的病毒：人冠状病毒 HKU1 可在人纤毛气道上皮细胞培养物中感染、复制并产生子代病毒颗粒。
J Virol. 2010 Nov;84(21):11255-63. doi: 10.1128/JVI.00947-10. Epub 2010 Aug 18.
6
Determining the Replication Kinetics and Cellular Tropism of Influenza D Virus on Primary Well-Differentiated Human Airway Epithelial Cells.测定原发性人呼吸道上皮细胞中 D 型流感病毒的复制动力学和细胞嗜性。
Viruses. 2019 Apr 24;11(4):377. doi: 10.3390/v11040377.
7
Establishment of caprine airway epithelial cells grown in an air-liquid interface system to study caprine respiratory viruses and bacteria.建立山羊气道上皮细胞在气液界面培养系统中研究山羊呼吸道病毒和细菌。
Vet Microbiol. 2021 Jun;257:109067. doi: 10.1016/j.vetmic.2021.109067. Epub 2021 Apr 10.
8
Culturing of respiratory viruses in well-differentiated pseudostratified human airway epithelium as a tool to detect unknown viruses.在高度分化的假复层人呼吸道上皮中培养呼吸道病毒作为检测未知病毒的一种工具。
Influenza Other Respir Viruses. 2015 Jan;9(1):51-7. doi: 10.1111/irv.12297. Epub 2014 Dec 8.
9
Differentiated swine airway epithelial cell cultures for the investigation of influenza A virus infection and replication.用于流感 A 病毒感染和复制研究的分化猪呼吸道上皮细胞培养。
Influenza Other Respir Viruses. 2013 Mar;7(2):139-50. doi: 10.1111/j.1750-2659.2012.00371.x. Epub 2012 Apr 25.
10
Ferret airway epithelial cell cultures support efficient replication of influenza B virus but not mumps virus.雪貂气道上皮细胞培养物支持乙型流感病毒高效复制，但不支持腮腺炎病毒复制。
J Gen Virol. 2015 Aug;96(8):2092-2098. doi: 10.1099/vir.0.000176. Epub 2015 May 6.
引用本文的文献
1
The Pestivirus RNase E Tames the Interferon Response of the Respiratory Epithelium.瘟病毒核糖核酸酶E抑制呼吸道上皮细胞的干扰素反应。
Viruses. 2024 Dec 11;16(12):1908. doi: 10.3390/v16121908.
2
A clinical protocol for a German birth cohort study of the Maturation of Immunity Against respiratory viral Infections (MIAI).一项关于呼吸道病毒感染免疫成熟（MIAI）的德国出生队列研究的临床方案。
Front Immunol. 2024 Sep 17;15:1443665. doi: 10.3389/fimmu.2024.1443665. eCollection 2024.
3
Drug repurposing screen identifies lonafarnib as respiratory syncytial virus fusion protein inhibitor.药物重定位筛选发现 lonafarnib 是呼吸道合胞病毒融合蛋白抑制剂。
Nat Commun. 2024 Feb 8;15(1):1173. doi: 10.1038/s41467-024-45241-y.
4
Repurposing screen identifies novel candidates for broad-spectrum coronavirus antivirals and druggable host targets.重新利用筛选方法识别广谱冠状病毒抗病毒药物和可用药的宿主靶点的新型候选药物。
Antimicrob Agents Chemother. 2024 Mar 6;68(3):e0121023. doi: 10.1128/aac.01210-23. Epub 2024 Feb 6.
5
Respiratory Syncytial Virus Two-Step Infection Screen Reveals Inhibitors of Early and Late Life Cycle Stages.呼吸道合胞病毒两步感染筛选揭示早期和晚期生命周期阶段的抑制剂。
Antimicrob Agents Chemother. 2022 Dec 20;66(12):e0103222. doi: 10.1128/aac.01032-22. Epub 2022 Nov 8.
6
Comprehensive single cell analysis of pandemic influenza A virus infection in the human airways uncovers cell-type specific host transcriptional signatures relevant for disease progression and pathogenesis.全面的人类呼吸道内大流行性流感病毒感染的单细胞分析揭示了与疾病进展和发病机制相关的特定细胞类型的宿主转录特征。
Front Immunol. 2022 Oct 4;13:978824. doi: 10.3389/fimmu.2022.978824. eCollection 2022.
7
Modeling SARS-CoV-2 and influenza infections and antiviral treatments in human lung epithelial tissue equivalents.模拟 SARS-CoV-2 和流感病毒感染及在人肺上皮组织等效物中抗 病毒治疗。
Commun Biol. 2022 Aug 12;5(1):810. doi: 10.1038/s42003-022-03753-7.
8
Responses of reconstituted human bronchial epithelia from normal and health-compromised donors to non-volatile particulate matter emissions from an aircraft turbofan engine.重建的来自正常和健康受损供体的人支气管上皮对飞机涡扇发动机排放的非挥发性颗粒物的反应。
Environ Pollut. 2022 Aug 15;307:119521. doi: 10.1016/j.envpol.2022.119521. Epub 2022 May 24.
9
Effective Interferon Lambda Treatment Regimen To Control Lethal MERS-CoV Infection in Mice.有效干扰素 λ 治疗方案可控制小鼠致命性中东呼吸综合征冠状病毒感染。
J Virol. 2022 Jun 8;96(11):e0036422. doi: 10.1128/jvi.00364-22. Epub 2022 May 19.
10
Human Organotypic Airway and Lung Organoid Cells of Bronchiolar and Alveolar Differentiation Are Permissive to Infection by Influenza and SARS-CoV-2 Respiratory Virus.人器官型气道和肺泡分化的肺类器官细胞允许流感和 SARS-CoV-2 呼吸道病毒感染。
Front Cell Infect Microbiol. 2022 Mar 14;12:841447. doi: 10.3389/fcimb.2022.841447. eCollection 2022.
本文引用的文献
1
Targeting membrane-bound viral RNA synthesis reveals potent inhibition of diverse coronaviruses including the middle East respiratory syndrome virus.靶向膜结合病毒RNA合成可有效抑制包括中东呼吸综合征病毒在内的多种冠状病毒。
PLoS Pathog. 2014 May 29;10(5):e1004166. doi: 10.1371/journal.ppat.1004166. eCollection 2014 May.
2
Interferon lambda 4 signals via the IFNλ receptor to regulate antiviral activity against HCV and coronaviruses.干扰素 lambda 4 通过 IFNλ 受体发出信号，调节针对 HCV 和冠状病毒的抗病毒活性。
EMBO J. 2013 Nov 27;32(23):3055-65. doi: 10.1038/emboj.2013.232. Epub 2013 Oct 29.
3
Isolation and characterization of current human coronavirus strains in primary human epithelial cell cultures reveal differences in target cell tropism.在原代人上皮细胞培养物中分离和鉴定当前人类冠状病毒株揭示了靶细胞嗜性的差异。
J Virol. 2013 Jun;87(11):6081-90. doi: 10.1128/JVI.03368-12. Epub 2013 Feb 20.
4
Efficient replication of the novel human betacoronavirus EMC on primary human epithelium highlights its zoonotic potential.新型人类贝塔冠状病毒 EMC 在原代人上皮细胞中的有效复制突出了其人畜共患病的潜力。
mBio. 2013 Feb 19;4(1):e00611-12. doi: 10.1128/mBio.00611-12.
5
Culturing the unculturable: human coronavirus HKU1 infects, replicates, and produces progeny virions in human ciliated airway epithelial cell cultures.培养无法培养的病毒：人冠状病毒 HKU1 可在人纤毛气道上皮细胞培养物中感染、复制并产生子代病毒颗粒。
J Virol. 2010 Nov;84(21):11255-63. doi: 10.1128/JVI.00947-10. Epub 2010 Aug 18.
6
Air-liquid interface (ALI) culture of human bronchial epithelial cell monolayers as an in vitro model for airway drug transport studies.人支气管上皮细胞单层的气液界面（ALI）培养作为气道药物转运研究的体外模型。
J Pharm Sci. 2007 Feb;96(2):341-50. doi: 10.1002/jps.20803.
7
Identification of cell lines permissive for human coronavirus NL63.鉴定对人冠状病毒NL63易感的细胞系。
J Virol Methods. 2006 Dec;138(1-2):207-10. doi: 10.1016/j.jviromet.2006.07.023. Epub 2006 Sep 7.
8
Infection of human airway epithelium by human and avian strains of influenza a virus.甲型流感病毒的人源和禽源毒株对人呼吸道上皮的感染。
J Virol. 2006 Aug;80(16):8060-8. doi: 10.1128/JVI.00384-06.
9
Development of one-step, real-time, quantitative reverse transcriptase PCR assays for absolute quantitation of human coronaviruses OC43 and 229E.用于绝对定量人冠状病毒OC43和229E的一步法实时定量逆转录PCR检测方法的开发。
J Clin Microbiol. 2005 Nov;43(11):5452-6. doi: 10.1128/JCM.43.11.5452-5456.2005.
10
Well-differentiated human airway epithelial cell cultures.高分化人呼吸道上皮细胞培养物
Methods Mol Med. 2005;107:183-206. doi: 10.1385/1-59259-861-7:183.
Zotero 插件