Muzzin P, Revelli J P, Ricquier D, Meier M K, Assimacopoulos-Jeannet F, Giacobino J P
Département de Biochimie médicale, Centre médicale Universitaire, Genève, Switzerland.
Biochem J. 1989 Aug 1;261(3):721-4. doi: 10.1042/bj2610721.
In obese (fa/fa) rats both the total (-)-isoprenaline- and NaF-stimulated adenylate cyclase activities of interscapular brown-adipose-tissue (IBAT) plasma membranes were decreased as compared with lean rats by 40 and 38% respectively. An acute treatment with Ro 16-8714 increased (-)-isoprenaline- and NaF-stimulated adenylate cyclase activities by 2.5- and 2.0-fold respectively, and beta-adrenoceptor number 2.8-fold in obese rat IBAT, but it had no effect on these parameters in lean rats. It increased mRNA for mitochondrial uncoupling protein in both lean and obese rats to the same extent.
与瘦大鼠相比,肥胖(fa/fa)大鼠肩胛间棕色脂肪组织(IBAT)质膜中,总的(-)-异丙肾上腺素和氟化钠刺激的腺苷酸环化酶活性分别降低了40%和38%。用Ro 16-8714进行急性处理后,肥胖大鼠IBAT中(-)-异丙肾上腺素和氟化钠刺激的腺苷酸环化酶活性分别增加了2.5倍和2.0倍,β-肾上腺素能受体数量增加了2.8倍,但对瘦大鼠的这些参数没有影响。它使瘦大鼠和肥胖大鼠线粒体解偶联蛋白的mRNA增加到相同程度。