PCR 电喷雾电离质谱技术在人体组织中毛霉目真菌的分子鉴定中的应用。
Molecular identification of Mucorales in human tissues: contribution of PCR electrospray-ionization mass spectrometry.
机构信息
Laboratoire de Parasitologie-Mycologie, AP-HP, Groupe Hospitalier Saint-Louis-Lariboisière-Fernand-Widal, Paris, France; Université Paris Diderot, Sorbonne Paris Cité, Paris, France; Institut Pasteur, Unité de Mycologie Moléculaire, Centre National de Référence Mycoses Invasives et Antifongiques, Paris, France; CNRS URA3012, Paris, France.
Institut Pasteur, Unité de Mycologie Moléculaire, Centre National de Référence Mycoses Invasives et Antifongiques, Paris, France; CNRS URA3012, Paris, France.
出版信息
Clin Microbiol Infect. 2015 Jun;21(6):594.e1-5. doi: 10.1016/j.cmi.2015.01.017. Epub 2015 Jan 28.
Molecular methods are crucial for mucormycosis diagnosis because cultures are frequently negative, even if microscopy suggests the presence of hyphae in tissues. We assessed PCR/electrospray-ionization mass spectrometry (PCR/ESI-MS) for Mucorales identification in 19 unfixed tissue samples from 13 patients with proven or probable mucormycosis and compared the results with culture, quantitative real-time PCR, 16S-23S rRNA gene internal transcribed spacer region (ITS PCR) and 18S PCR sequencing. Concordance with culture identification to both genus and species levels was higher for PCR/ESI-MS than for the other techniques. Thus, PCR/ESI-MS is suitable for Mucorales identification, within 6 hours, for tissue samples for which microscopy results suggest the presence of hyphae.
分子方法对于毛霉病的诊断至关重要，因为即使显微镜显示组织中有菌丝存在，培养也常常为阴性。我们评估了 PCR/电喷雾电离质谱（PCR/ESI-MS）在 13 例确诊或疑似毛霉病患者的 19 个未经固定的组织样本中的应用，将结果与培养、定量实时 PCR、16S-23S rRNA 基因内转录间隔区（ITS-PCR）和 18S-PCR 测序进行比较。与其他技术相比，PCR/ESI-MS 在属和种水平上与培养鉴定的一致性更高。因此，对于显微镜检查结果提示存在菌丝的组织样本，PCR/ESI-MS 适合在 6 小时内进行毛霉属鉴定。
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