Ma H-T, Jiang H-B, Liu X-Q, Wu X-P, Wei X-M
Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai, China.
Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai, China
Genet Mol Res. 2015 Jan 15;14(1):145-8. doi: 10.4238/2015.January.15.17.
The razor clam, Sinonovacula constricta, is an important commercial bivalve and a popular mollusca food in China. Twelve polymorphic microsatellite markers were isolated from the razor clam using a partial genomic library enriched for tandem repeat sequences of (CA)16, (GA)16. Polymorphisms of these loci were evaluated in a wild population of 30 individuals. The allele number of these polymorphic markers ranged from 5-15 per locus with an average of 9.333. Observed and expected heterozygosity values ranged from 0.192-1.000 and 0.219-0.906. Polymorphism information content ranged from 0.209-0.892 with an average of 0.704. Three loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. No significant linkage disequilibrium was detected between these loci. This set of microsatellite loci are useful for genetic studies in S. constricta.
缢蛏（Sinonovacula constricta）是一种重要的商业双壳贝类，也是中国受欢迎的软体动物食品。利用富含（CA）16、（GA）16串联重复序列的部分基因组文库，从缢蛏中分离出12个多态性微卫星标记。在30个个体的野生群体中评估了这些位点的多态性。这些多态性标记的等位基因数每个位点范围为5 - 15个，平均为9.333个。观察到的和预期的杂合度值范围分别为0.192 - 1.000和0.219 - 0.906。多态性信息含量范围为0.209 - 0.892，平均为0.704。经Bonferroni校正后，有3个位点显著偏离哈迪 - 温伯格平衡。在这些位点之间未检测到显著的连锁不平衡。这组微卫星位点可用于缢蛏的遗传学研究。
