Liu T A, Fang Y X, Yin H, Jing Z Z
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of Agricultural Ministry, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of Agricultural Ministry, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China
Genet Mol Res. 2015 Jan 26;14(1):507-14. doi: 10.4238/2015.January.26.4.
φC31 integrase encoded by Streptomyces phage can mediate site-specific recombination between phage and host genomes. The recombination direction is generally considered to be accurately regulated, but the regulatory mechanisms involved are still unclear. Recently, some hyperactive mutants of φC31 integrase that can bypass the regulatory steps have been isolated and extensively studied. A putative coiled-coil region is found to play a critical role in controlling recombination direction. Further analysis led us to the speculation that at least two regions in the N-terminal domain of φC31 integrase are involved in the tetrameric interfaces and that the putative coiled coil interacts with one of the regions to regulate the recombination direction.
由链霉菌噬菌体编码的φC31整合酶可介导噬菌体与宿主基因组之间的位点特异性重组。重组方向通常被认为受到精确调控，但其中涉及的调控机制仍不清楚。最近，一些能够绕过调控步骤的φC31整合酶的高活性突变体已被分离并进行了广泛研究。人们发现一个假定的卷曲螺旋区域在控制重组方向中起关键作用。进一步分析使我们推测，φC31整合酶N端结构域中至少有两个区域参与四聚体界面的形成，并且假定的卷曲螺旋与其中一个区域相互作用以调控重组方向。
