Liang Y, Yang X M, Gu Y R, Tao X, Zhong Z Z, Gong J J, Chen X H, Lv X B
Sichuan Animal Science Academy, Chengdu, Sichuan, China.
Sichuan Animal Science Academy, Chengdu, Sichuan, China
Genet Mol Res. 2015 Feb 13;14(1):1287-92. doi: 10.4238/2015.February.13.7.
Glucose transporter proteins 2 and 4 (GLUT2 and GLUT4) play important roles in glucose transport and energy metabolism. Changes in the levels of GLUT2 and GLUT4 mRNA were measured in longissimus dorsi muscle from the lean Yorkshire and fat Tibetan pig breeds at six different time points (1, 2, 3, 4, 5, and 6 months) with quantitative real-time polymerase chain reactions. The results showed that GLUT2 and GLUT4 mRNA were abundantly expressed in the longissimus dorsi muscle and that the developmental expression patterns were similar in both breeds. Tibetan pigs exhibited higher intramuscular fat and GLUT2 mRNA levels, while Yorkshire pigs exhibited a higher myofiber cross-sectional area (CSA) and GLUT4 mRNA levels. Furthermore, the changes in the GLUT4 mRNA levels were strongly and positively correlated with the CSA over a period of six months. These results exhibit time- and breed-specific expression patterns of GLUT2 and GLUT4, which highlight their potential as candidate genes for assessing adipose deposition and muscle development in pigs. These differences in the expression of GLUT family genes may also have indications for meat quality.
葡萄糖转运蛋白2和4（GLUT2和GLUT4）在葡萄糖转运和能量代谢中发挥着重要作用。利用定量实时聚合酶链反应，在六个不同时间点（1、2、3、4、5和6个月）测定了瘦肉型约克夏猪和脂肪型藏猪背最长肌中GLUT2和GLUT4 mRNA水平的变化。结果表明，GLUT2和GLUT4 mRNA在背最长肌中大量表达，且两个品种的发育表达模式相似。藏猪的肌内脂肪和GLUT2 mRNA水平较高，而约克夏猪的肌纤维横截面积（CSA）和GLUT4 mRNA水平较高。此外，在六个月的时间里，GLUT4 mRNA水平的变化与CSA呈强烈正相关。这些结果显示了GLUT2和GLUT4的时间和品种特异性表达模式，突出了它们作为评估猪脂肪沉积和肌肉发育候选基因的潜力。GLUT家族基因表达的这些差异也可能对肉质有指示作用。
