Kwon S-J, Yu J, Park Y J, Son J-H, Kim N-S, Lee J K
Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan, Korea.
Department of Molecular Bioscience, Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Korea.
Genet Mol Res. 2015 Feb 13;14(1):1347-61. doi: 10.4238/2015.February.13.14.
Seed shattering of wild plant species is thought to be an adaptive trait to facilitate seed dispersal. For rice breeding, seed shatter-ing is an important trait for improving breeding strategies, particularly when developing lines use interspecific hybrids and introgression of genes from wild species. We developed F₃:₄ recombinant inbred lines from an interspecific cross between Oryza sativa cv. Ilpoombyeo and Oryza rufipogon. In this study, we genetically analyzed known shat-tering-related loci using the F₃:₄ population of O. sativa/O. rufipogon. CACTA-AG190 was significantly associated with the shattering trait CACTA-TD according to bulked segregant analysis results, and was found in the qSH-1 region of chromosome 1. Fine genetic mapping of the flanking regions around qSH-1 based on CACTA-AG190 revealed multiple-sequence variations. The highest limit of detection based on quantitative trait locus analysis was observed between shaap-7715 and a 518-bp insertion site. Two other quantitative trait locus analyses of seed-shattering-related loci, qSH-4 and sh-h, were performed using simple sequence repeat and allele-pecific single nucleotide polymor-phism markers. Our results can be applied for rice-breeding research, such as marker-assisted selection between cultivated and wild rice.
野生植物物种的种子脱落被认为是一种促进种子传播的适应性性状。对于水稻育种而言，种子脱落是改进育种策略的一个重要性状，尤其是在培育利用种间杂种和野生种基因渗入的品系时。我们从栽培稻品种Ilpoombyeo与野生稻的种间杂交中培育出了F₃:₄重组自交系。在本研究中，我们利用栽培稻/野生稻的F₃:₄群体对已知的与脱落相关的位点进行了遗传分析。根据混合分组分析法的结果，CACTA - AG190与脱落性状CACTA - TD显著相关，且位于第1染色体的qSH - 1区域。基于CACTA - AG190对qSH - 1周围侧翼区域进行精细遗传定位，发现了多个序列变异。在shaap - 7715和一个518bp插入位点之间观察到基于数量性状基因座分析的最高检测极限。利用简单序列重复和等位基因特异性单核苷酸多态性标记对另外两个与种子脱落相关的位点qSH - 4和sh - h进行了数量性状基因座分析。我们的结果可应用于水稻育种研究，如栽培稻和野生稻之间的标记辅助选择。
