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微卫星和单核苷酸多态性标记在欧洲家猪亲子鉴定中的有效性

Effectiveness of microsatellite and single nucleotide polymorphism markers for parentage analysis in European domestic pigs.

作者信息

Yu G C, Tang Q Z, Long K R, Che T D, Li M Z, Shuai S R

机构信息

Institute of Animal Genetics & Breeding, College of Animal Science & Technology, Sichuan Agricultural University, Ya'an, China.

Institute of Animal Genetics & Breeding, College of Animal Science & Technology, Sichuan Agricultural University, Ya'an, China

出版信息

Genet Mol Res. 2015 Feb 13;14(1):1362-70. doi: 10.4238/2015.February.13.15.

Abstract

Parentage analysis and individual identification are recent, promising methods that have been applied to evolutionary and ecological studies, as well as conservation management. Parental exclusion relying on polymorphic microsatellites has been used worldwide in parentage determination, while the low mutation rate and genotyping error rate of single nucleotide polymorphisms (SNPs) make them another important marker for pedigree tracing. Here, we compared the effectiveness of microsatellites and SNP markers in European pigs. We also measured and presented the minimum and optimal criteria for SNP markers to be used in paternity and identity analysis. Our findings may contribute to the development of techniques for future molecular evolution and conservation studies, as well as breeding programs.

摘要

亲权分析和个体识别是最近应用于进化和生态研究以及保护管理的很有前景的方法。依靠多态微卫星的亲权排除法已在全球范围内用于亲权鉴定,而单核苷酸多态性(SNP)的低突变率和基因分型错误率使其成为谱系追踪的另一个重要标记。在此,我们比较了微卫星和SNP标记在欧洲猪中的有效性。我们还测量并提出了SNP标记用于父权和身份分析的最低和最佳标准。我们的研究结果可能有助于未来分子进化和保护研究以及育种计划的技术发展。

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