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用于欧洲野猪(Sus scrofa)鉴定和亲子关系分析的微卫星标记。

Microsatellite markers for identification and parentage analysis in the European wild boar (Sus scrofa).

作者信息

Costa Vânia, Pérez-González Javier, Santos Pedro, Fernández-Llario Pedro, Carranza Juan, Zsolnai Attila, Anton István, Buzgó József, Varga Gyula, Monteiro Nuno, Beja-Pereira Albano

机构信息

Centro de Investigação em Biodiversidade e Recursos Genéticos da Universidade do Porto (CIBIO-UP), Rua Padre Armando Quintas 7, 4485-661 Vairão, Portugal.

出版信息

BMC Res Notes. 2012 Sep 3;5:479. doi: 10.1186/1756-0500-5-479.

Abstract

BACKGROUND

The wild boar (Sus scrofa) is among the most widespread mammal species throughout the old world. Presently, studies concerning microsatellites in domestic pigs and wild boars have been carried out in order to investigate domestication, social behavior and general diversity patterns among either populations or breeds. The purpose of the current study is to develop a robust set of microsatellites markers for parentage analyses and individual identification.

FINDINGS

A set of 14 previously reported microsatellites markers have been optimized and tested in three populations from Hungary, Portugal and Spain, in a total of 167 samples. The results indicate high probabilities of exclusion (0.99999), low probability of identity (2.0E(-13) - 2.5E(-9)) and a parentage assignment of 100%.

CONCLUSIONS

Our results demonstrate that this set of markers is a useful and efficient tool for the individual identification and parentage assignment in wild boars.

摘要

背景

野猪(Sus scrofa)是旧世界中分布最广的哺乳动物物种之一。目前,已经开展了关于家猪和野猪微卫星的研究,以调查种群或品种之间的驯化、社会行为和一般多样性模式。本研究的目的是开发一套强大的微卫星标记用于亲子鉴定和个体识别。

研究结果

一组14个先前报道的微卫星标记在来自匈牙利、葡萄牙和西班牙的三个种群的总共167个样本中进行了优化和测试。结果表明排除概率高(0.99999),身份概率低(2.0E(-13) - 2.5E(-9)),亲子鉴定成功率为100%。

结论

我们的结果表明,这套标记是野猪个体识别和亲子鉴定的有用且高效的工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b50/3475110/e6339d34d212/1756-0500-5-479-1.jpg

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