Romaniuk O P, Nikitchenko N V, Savina N V, Kuzhir T D, Goncharova R I
Genetika. 2014 Aug;50(8):975-85.
The polymorphism of excision repair genes XPD Asp312Asn, XRCC1 Arg399Gln, OGG1 Ser326Cys, and ERCC6 Met1097Val was analyzed by PCR-RFLP in 370 representatives of the Belarusian population of average, old, and elderly ages. Correlation analysis showed that the frequencies of wild-type homozygous combinations significantly increase with age in the group of subjects over 70 years old in the case of the interaction of two genes, XPD 312 and XRCC1399, or three genes, XPD312, XRCC1399, and ERCC6 1097. In a subgroup of the long-lived, this relationship is manifested in case of a pairwise interaction of gene XPD 312 with XRCC1 399 or ERCC6 1097, as well as an interaction of three genes, XPD 312, XRCC1 399, and ERCC6 1097. The data suggest that the optimum activity of repair processes may favor longevity. It is shown that the frequency of the Asp/Asp genotype is reduced, and the frequency of the Asn allele of the XPD 312 gene is increased in the subgroup of smokers as compared with nonsmokers, which apparently indicates an association of this gene polymorphism with an inclination to smoke. The problem requires further study.
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术，对白俄罗斯平均年龄、老年和高龄人群的370名代表进行了切除修复基因XPD Asp312Asn、XRCC1 Arg399Gln、OGG1 Ser326Cys和ERCC6 Met1097Val多态性分析。相关性分析表明，在70岁以上的受试者组中，当两个基因XPD 312和XRCC1399或三个基因XPD312、XRCC1399和ERCC6 1097相互作用时，野生型纯合组合的频率随年龄显著增加。在长寿亚组中，这种关系在基因XPD 312与XRCC1 399或ERCC6 1097的成对相互作用以及三个基因XPD 312、XRCC1 399和ERCC6 1097的相互作用中表现出来。数据表明，修复过程的最佳活性可能有利于长寿。结果显示，与不吸烟者相比，吸烟者亚组中XPD 312基因的Asp/Asp基因型频率降低，Asn等位基因频率增加，这显然表明该基因多态性与吸烟倾向有关。该问题需要进一步研究。
