苏云金芽孢杆菌Cry1蛋白在甘蔗螟虫（Diatraea saccharalis，鳞翅目：草螟科）中的结合分析
Binding analysis of Bacillus thuringiensis Cry1 proteins in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae).
作者信息
Davolos Camila C, Hernández-Martinez Patricia, Crialesi-Legori Paula C B, Desidério Janete A, Ferré Juan, Escriche Baltasar, Lemos Manoel Victor F
机构信息
Univ Estadual Paulista "Júlio de Mesquita Filho" UNESP/FCAV, Department of Applied Biology, Rod Prof. Paulo Donato Castellane km 5, CEP 14884-900, Jaboticabal, São Paulo, Brazil; Departamento de Genética, Facultad de CC. Biológicas, Universidad de Valencia, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.
Departamento de Genética, Facultad de CC. Biológicas, Universidad de Valencia, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.
出版信息
J Invertebr Pathol. 2015 May;127:32-4. doi: 10.1016/j.jip.2015.01.013. Epub 2015 Feb 28.
Sugarcane borer (Diatraea saccharalis, F.) is an important corn pest in South America and United States. The aim of the present study was to analyze the susceptibility and binding interactions of three Cry1A proteins and Cry1Fa in a Brazilian D. saccharalis population. The results showed that Cry1Ab was the most active, followed by Cry1Ac, Cry1Fa and Cry1Aa. All Cry1-biotinylated proteins tested bound specifically to the D. saccharalis brush border membrane vesicles (BBMV). Heterologous competition assays showed shared binding sites for all Cry1A proteins and another one shared by Cry1Fa and Cry1Ab. Thus, pyramiding Cry1Aa/Cry1Ac and Cry1F proteins would be a recommended strategy for managing this pest.
甘蔗螟虫（Diatraea saccharalis, F.）是南美洲和美国一种重要的玉米害虫。本研究的目的是分析三种Cry1A蛋白和Cry1Fa对巴西甘蔗螟虫种群的敏感性及结合相互作用。结果表明，Cry1Ab活性最高，其次是Cry1Ac、Cry1Fa和Cry1Aa。所有测试的Cry1-生物素化蛋白都能特异性结合甘蔗螟虫的刷状缘膜囊泡（BBMV）。异源竞争试验表明，所有Cry1A蛋白具有共同的结合位点，Cry1Fa和Cry1Ab具有另一个共同结合位点。因此，将Cry1Aa/Cry1Ac和Cry1F蛋白进行基因叠加是治理这种害虫的推荐策略。
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