高度多态性位点D16S7的群体遗传学及其在亲子鉴定中的应用。

Chimera J A, Harris C R, Litt M

Department of Molecular Genetics, Roche Biomedical Laboratories, Incorporated, Burlington, NC 27215.

Am J Hum Genet. 1989 Dec;45(6):926-31.

We have developed a DNA RFLP test to resolve paternity cases in which the accused man is included at a low probability of paternity by conventional testing. The DNA probe p79-2-23 was used to determine the allele frequency distribution for the locus D16S7 in the North American black, Caucasian, and Hispanic racial groups. Approximately 3,500 TaqI-digested DNAs were analyzed from the three populations studied. An apparent continuum of alleles was detected varying in size from 2.9 kb to 8.3 kb. Estimates of the average probability of exclusion were found to be .90 and .79 for the North American black and Caucasian populations, respectively. Gene frequency data for common and rare alleles indicated a potential paternity index ranging from 2 to 450.

我们开发了一种DNA限制性片段长度多态性（RFLP）检测方法，用于解决那些通过传统检测认定被指控男子为父亲的可能性较低的亲子鉴定案件。DNA探针p79 - 2 - 23被用于确定北美黑人、白种人和西班牙裔种族群体中D16S7基因座的等位基因频率分布。对所研究的三个群体中约3500份经TaqI酶切的DNA进行了分析。检测到大小从2.9 kb到8.3 kb不等的明显连续等位基因。发现北美黑人和白种人群体的平均排除概率估计分别为0.90和0.79。常见和罕见等位基因的基因频率数据表明，潜在的父权指数范围为2至450。