Kant Ravi, Sharma Shweta, Dasgupta Indranil
Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.
Methods Mol Biol. 2015;1287:201-17. doi: 10.1007/978-1-4939-2453-0_15.
The large-scale functional analysis of genes in plants depends heavily on robust techniques for gene silencing. Virus-induced gene silencing (VIGS) is a transient gene silencing method for plants, triggered by the inoculation of a modified viral vector carrying a fragment of the gene targeted for silencing. Here we describe a VIGS protocol for rice, based on the Rice tungro bacilliform virus (RTBV, a DNA virus). We present an updated and detailed protocol for silencing of the gene encoding Phytoene desaturase in rice, using the RTBV-VIGS system.
植物基因的大规模功能分析在很大程度上依赖于强大的基因沉默技术。病毒诱导的基因沉默（VIGS）是一种针对植物的瞬时基因沉默方法，通过接种携带靶向沉默基因片段的改良病毒载体来触发。在此，我们描述了一种基于水稻东格鲁杆状病毒（RTBV，一种DNA病毒）的水稻VIGS方案。我们使用RTBV-VIGS系统，给出了一个更新的、详细的水稻八氢番茄红素去饱和酶编码基因沉默方案。
