Simon Hans-Georg, Odelberg Shannon
Department of Pediatrics, Lurie Children's Hospital of Chicago Research Center, Northwestern University Feinberg School of Medicine, 225 E. Chicago Avenue, Box 204, Chicago, IL, 60611, USA,
Methods Mol Biol. 2015;1290:17-25. doi: 10.1007/978-1-4939-2495-0_2.
The adult Eastern newt, Notophthalmus viridescens, has long served as a model for appendage as well as heart muscle regeneration studies. Newt tissues include all major cell types known in other vertebrates and mammals, including bone, cartilage, tendon, muscle, nerves, dermis, and epidermis. Therefore, these aquatic salamanders make an excellent model for studying the regeneration of complex tissues. Regeneration of adult tissues requires the integration of new tissues with preexisting tissues to form a functioning unit through a process that is not yet well understood. Scale is also an issue, because the regenerating tissues or structures are magnitudes larger than their embryonic counterparts during development, and therefore, it is likely that different physics and mechanics apply. Regardless, regeneration recapitulates to some degree developmental processes. In this chapter, we will describe basic methods for maintaining adult Eastern newts in the laboratory for the study of regeneration. To determine similarities and differences between development and regeneration at the cellular and molecular level, there is also a need for embryonic newt tissue. We therefore also outline a relatively simple way to produce and raise newt embryos in the laboratory.
成年东部蝾螈（Notophthalmus viridescens）长期以来一直是附肢以及心肌再生研究的模型。蝾螈组织包括其他脊椎动物和哺乳动物中已知的所有主要细胞类型，包括骨骼、软骨、肌腱、肌肉、神经、真皮和表皮。因此，这些水生蝾螈是研究复杂组织再生的极佳模型。成体组织的再生需要新组织与原有组织整合，通过一个尚未完全理解的过程形成一个功能单元。规模也是一个问题，因为再生组织或结构比其发育过程中的胚胎对应物大得多，因此，可能适用不同的物理和力学原理。无论如何，再生在某种程度上重现了发育过程。在本章中，我们将描述在实验室中饲养成年东部蝾螈以进行再生研究的基本方法。为了在细胞和分子水平上确定发育与再生之间的异同，还需要胚胎蝾螈组织。因此，我们还概述了一种在实验室中生产和饲养蝾螈胚胎的相对简单的方法。
