通过蝾螈胚胎联体共生产生无神经幼虫。

Generation of aneurogenic larvae by parabiosis of salamander embryos.

作者信息

Kumar Anoop, Delgado Jean Paul

机构信息

Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London, WC1E 6BT, UK,

出版信息

Methods Mol Biol. 2015;1290:147-57. doi: 10.1007/978-1-4939-2495-0_12.

DOI:10.1007/978-1-4939-2495-0_12

PMID:25740484

Abstract

Limb regeneration of salamanders is nerve dependent, and the removal of the nerves in early stages of limb regeneration severely curtails the proliferation of the blastemal cells and growth of the regenerate. The removal of the neural tube from a developing salamander embryo results in an aneurogenic larva and the aneurogenic limb (ANL) develops independently without innervation. Paradoxically, the limb in an ANL is capable of regeneration in a nerve-independent manner. Here, we describe a detailed method for the generation of ANL in the spotted salamander, Ambystoma maculatum, for regeneration studies.

摘要

蝾螈的肢体再生依赖于神经，在肢体再生早期去除神经会严重抑制芽基细胞的增殖和再生肢体的生长。从发育中的蝾螈胚胎中移除神经管会导致无神经幼虫，无神经肢体（ANL）在没有神经支配的情况下独立发育。矛盾的是，ANL中的肢体能够以不依赖神经的方式再生。在这里，我们描述了一种在黄斑蝾螈（Ambystoma maculatum）中生成ANL以进行再生研究的详细方法。