二苯胺-2-羧酸盐对牛气管上皮细胞钠和氯转运的影响：单层细胞和天然组织的研究

Effects of diphenylamine-2-carboxylate on sodium and chloride transport across bovine tracheal epithelium: studies in monolayers and in the native tissue.

Durand J

Institute of Physiology, University of Fribourg, Switzerland.

Comp Biochem Physiol A Comp Physiol. 1989;94(3):447-53. doi: 10.1016/0300-9629(89)90119-9.

Abstract

The short-circuit current (I0) across monolayers of bovine tracheal epithelial cells is the sum of Na+ absorption and Cl- secretion. 2. Diphenylamine-2-carboxylate (DPC), added to the mucosal side of the native tissue or monolayers induced a rapid, dose-dependent and fully reversible reduction in I0, which reached zero with 3 mM DPC. 3. The blocking effect of DPC was examined during incubation (1) in standard, (2) in Cl(-)-free and (3) in Na+-free solution. Dose response curves revealed that the IC50 was not altered following ion substitution: it was approximately 0.7 mM DPC. 4. Thus, in bovine tracheal epithelium, DPC was an effective blocking agent of both Na+ and Cl- transport.

摘要

跨牛气管上皮细胞单层的短路电流（I0）是Na+吸收和Cl-分泌的总和。2. 将二苯胺-2-羧酸盐（DPC）添加到天然组织或单层的黏膜侧，可诱导I0迅速、剂量依赖性且完全可逆地降低，在3 mM DPC时I0降至零。3. 在（1）标准溶液、（2）无Cl-溶液和（3）无Na+溶液中孵育期间检测了DPC的阻断作用。剂量反应曲线显示，离子替代后IC50未改变：约为0.7 mM DPC。4. 因此，在牛气管上皮中，DPC是Na+和Cl-转运的有效阻断剂。