Schuurman H J, Gmelig Meyling F H, Wijngaard P L, Van der Meulen A, Slootweg P J, Jambroes G
Department of Internal Medicine, University Hospital, Utrecht, The Netherlands.
J Pathol. 1989 Nov;159(3):197-203. doi: 10.1002/path.1711590305.
We performed immunological phenotyping of mononuclear cells in tissue sections of 84 endomyocardial biopsies (EMB) showing infiltrates, which were taken from 21 patients after heart transplantation. Data were correlated with histology (grading following Billingham) and cyto-immunologic monitoring (CIM) on blood samples (grading into negative, rejection, or infection, based on leukocyte morphology and T-cell phenotype). Few T lymphocytes were observed in 35 biopsies, and many in 49 biopsies. The semi-quantitative estimate of T cells and CD4/CD8 ratio did not correlate with EMB histology but was related to CIM data. For example, 31 out of 42 cases with a CIM indicative of infection (14 of which showing no rejection on histology) manifested large numbers of T cells. In most cases, the CD4/CD8 ratio was less than 1. The presence of activated cells (bearing interleukin-2 receptors or the CD30 antigen) was not related to EMB histology or to CIM data. The number of T cells (subsets) in EMB was not related to relative or absolute numbers in the blood.
我们对84份显示有浸润的心肌内膜活检（EMB）组织切片中的单核细胞进行了免疫表型分析，这些活检样本取自21例心脏移植后的患者。数据与组织学（按照比林厄姆标准分级）以及血样的细胞免疫监测（CIM，根据白细胞形态和T细胞表型分为阴性、排斥或感染）相关。在35份活检样本中观察到少量T淋巴细胞，在49份活检样本中观察到大量T淋巴细胞。T细胞和CD4/CD8比值的半定量评估与EMB组织学无关，但与CIM数据相关。例如，在42例CIM提示感染的病例中（其中14例组织学显示无排斥反应），有31例表现为大量T细胞。在大多数情况下，CD4/CD8比值小于1。活化细胞（携带白细胞介素-2受体或CD30抗原）的存在与EMB组织学或CIM数据无关。EMB中T细胞（亚群）的数量与血液中的相对或绝对数量无关。
