Souza Wallace Rafael, Silva Rafael Eduardo, Goodfellow Michael, Busarakam Kanungnid, Figueiro Fernanda Sales, Ferreira Douglas, Rodrigues-Filho Edson, Moraes Luiz Alberto Beraldo, Zucchi Tiago Domingues
Laboratório de Microbiologia Ambiental, Rod SP 340-Km 127, 5, Caixa Postal 69, EMBRAPA Meio Ambiente, 13820-000 Jaguariúna, Brazil.
School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
Int J Syst Evol Microbiol. 2015 Jun;65(Pt 6):1786-1793. doi: 10.1099/ijs.0.000176. Epub 2015 Mar 5.
Strain SB026T was isolated from Brazilian rainforest soil and its taxonomic position established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological features consistent with its classification in the genus Amycolatopsis and formed a branch in the Amycolatopsis 16S rRNA gene tree together with Amycolatopsis bullii NRRL B-24847T, Amycolatopsis plumensis NRRL B-24324T, Amycolatopsis tolypomycina DSM 44544T and Amycolatopsis vancoresmycina NRRL B-24208T. It was related most closely to A. bullii NRRL B-24847T (99.0 % 16S rRNA gene sequence similarity), but was distinguished from this strain by a low level of DNA-DNA relatedness (~46 %) and discriminatory phenotypic properties. Based on the combined genotypic and phenotypic data, it is proposed that the isolate should be classified in the genus Amycolatopsis as representing a novel species, Amycolatopsis rhabdoformis sp. nov. The type strain is SB026T ( = CBMAI 1694T = CMAA 1285T = NCIMB 14900T).
菌株SB026T从巴西雨林土壤中分离得到，利用多相研究数据确定其分类地位。该菌株表现出的化学分类和形态学特征组合与拟无枝酸菌属的分类一致，并且在拟无枝酸菌16S rRNA基因树中与布氏拟无枝酸菌NRRL B - 24847T、羽生拟无枝酸菌NRRL B - 24324T、托利拟无枝酸菌DSM 44544T和万氏拟无枝酸菌NRRL B - 24208T共同形成一个分支。它与布氏拟无枝酸菌NRRL B - 24847T关系最为密切（16S rRNA基因序列相似性为99.0%），但通过低水平的DNA - DNA相关性（约46%）和鉴别性表型特征与该菌株区分开来。基于综合的基因型和表型数据，建议将该分离株归为拟无枝酸菌属的一个新物种，命名为横纹拟无枝酸菌（Amycolatopsis rhabdoformis sp. nov.）。模式菌株为SB026T（= CBMAI 1694T = CMAA 1285T = NCIMB 14900T）。
