School of Biology, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK.
Antonie Van Leeuwenhoek. 2012 Jun;102(1):91-8. doi: 10.1007/s10482-012-9716-0. Epub 2012 Mar 21.
The status of two mesophilic filamentous actinomycetes isolated from an arid Australian soil sample was determined using a polyphasic taxonomic approach. The isolates had chemical and morphological properties consistent with their classification in the genus Amycolatopsis, assignments that were supported by analysis of 16S rRNA gene sequence data. Isolate SF26(T) formed a distinct phyletic line and hence was sharply separated from its nearest phylogenetic neighbour, Amycolatopsis sacchari DSM 44468(T). In contrast, isolate SF27(T) formed a subclade in the Amycolatopsis tree with Amycolatopsis vancoresmycina DSM 44592(T) but was separated readily from the latter by DNA:DNA pairing data. The two isolates were distinguished from one another and from their respective nearest phylogenetic neighbours using a range of phenotypic properties. These data indicate that the two isolates should be recognized as new species in the genus Amycolatopsis. The names proposed for these new taxa are Amycolatopsis bartoniae sp. nov. and Amycolatopsis bullii sp. nov. with isolates SF26(T) (=NCIMB 14706(T) = NRRL B-2846(T)) and SF27(T) (=NCIMB 14707(T) = NRRL B-24847(T)) as the respective type strains.
采用多相分类方法确定了从澳大利亚干旱土壤样本中分离出的两种嗜温丝状放线菌的地位。这些分离物的化学和形态特性与其在 Amycolatopsis 属中的分类一致，这一分类得到了 16S rRNA 基因序列数据分析的支持。分离株 SF26(T)形成了一个独特的系统发育线，因此与最接近的亲缘关系的 Amycolatopsis sacchari DSM 44468(T)明显分离。相比之下，分离株 SF27(T)在 Amycolatopsis 树中形成了一个亚分支，与 Amycolatopsis vancoresmycina DSM 44592(T) 相关，但通过 DNA:DNA 配对数据很容易与后者区分开来。这两个分离物通过一系列表型特性与彼此以及各自最近的亲缘关系的分类群区分开来。这些数据表明，这两个分离物应该被确认为 Amycolatopsis 属中的新种。提议的这两个新分类群的名称分别是 Amycolatopsis bartoniae sp. nov. 和 Amycolatopsis bullii sp. nov.，相应的模式株为 SF26(T) (=NCIMB 14706(T) = NRRL B-2846(T)) 和 SF27(T) (=NCIMB 14707(T) = NRRL B-24847(T))。
