新型聚（甲基丙烯酸氨基酸酯）刷支持形成结构明确的脂质膜。
New poly(amino acid methacrylate) brush supports the formation of well-defined lipid membranes.
作者信息
Blakeston Anita C, Alswieleh Abdullah M, Heath George R, Roth Johannes S, Bao Peng, Cheng Nan, Armes Steven P, Leggett Graham J, Bushby Richard J, Evans Stephen D
机构信息
†Molecular and Nanoscale Physics Group, School of Physics and Astronomy, University of Leeds, Leeds LS2 9JT, United Kingdom.
‡Department of Chemistry, University of Sheffield, Sheffield S3 7HF, United Kingdom.
出版信息
Langmuir. 2015 Mar 31;31(12):3668-77. doi: 10.1021/la504163s. Epub 2015 Mar 19.
A novel poly(amino acid methacrylate) brush comprising zwitterionic cysteine groups (PCysMA) was utilized as a support for lipid bilayers. The polymer brush provides a 12-nm-thick cushion between the underlying hard support and the aqueous phase. At neutral pH, the zeta potential of the PCysMA brush was ∼-10 mV. Cationic vesicles containing >25% DOTAP were found to form a homogeneous lipid bilayer, as determined by a combination of surface analytical techniques. The lipid mobility as measured by FRAP (fluorescence recovery after photobleaching) gave diffusion coefficients of ∼1.5 μm(2) s(-1), which are comparable to those observed for lipid bilayers on glass substrates.
一种包含两性离子半胱氨酸基团的新型聚（甲基丙烯酸氨基酸酯）刷（PCysMA）被用作脂质双层的支撑物。该聚合物刷在下层硬支撑物和水相之间提供了一个12纳米厚的缓冲层。在中性pH值下，PCysMA刷的zeta电位约为-10 mV。通过表面分析技术的组合确定，含有>25% DOTAP的阳离子囊泡形成了均匀的脂质双层。通过荧光漂白后恢复（FRAP）测量的脂质流动性给出了约1.5 μm² s⁻¹的扩散系数，这与在玻璃基板上观察到的脂质双层的扩散系数相当。
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