Macroporous silica particles derivatized for enhanced lectin affinity enrichment of glycoproteins.
作者信息
Mann Benjamin F
机构信息
Merck & Co., 126 E. Lincoln Avenue, 2000, Rahway, NJ, 07090, USA,
出版信息
Methods Mol Biol. 2015;1286:109-19. doi: 10.1007/978-1-4939-2447-9_10.
PMID:25749950
Abstract
This chapter details procedures for (1) functionalizing macroporous silica particles with lectins, a class of proteins that have affinity for the glycan moieties on glycoproteins, and (2) utilizing the lectin-silica material for high-performance affinity chromatography (HPAC) to enrich glycoproteins from small volumes of biological sample materials.
摘要
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