Chang H T, Kuo H
Department of Anatomy and Neurobiology, University of Tennessee, Memphis 38163.
Brain Res. 1989 Dec 4;503(2):350-3. doi: 10.1016/0006-8993(89)91690-9.
Adrenergic input to the rat substantia innominata (SI) was studied by immunocytochemical localization of phenylethanolamine N-methyltransferase (PNMT), the synthetic enzyme for adrenaline. Using primary antibodies derived from different species, we demonstrated in double-immunofluorescence labeling experiments that all PNMT-immunoreactive fibers in SI are also immunoreactive for tyrosine hydroxylase, the synthetic enzyme for dopamine. This result indicates that the PNMT immunoreactive axons in SI are indeed adrenergic fibers.
通过对肾上腺素合成酶苯乙醇胺N-甲基转移酶（PNMT）进行免疫细胞化学定位，研究了大鼠无名质（SI）的肾上腺素能输入。在双重免疫荧光标记实验中，我们使用来自不同物种的一抗，证明了SI中所有PNMT免疫反应性纤维对多巴胺合成酶酪氨酸羟化酶也具有免疫反应性。这一结果表明，SI中PNMT免疫反应性轴突确实是肾上腺素能纤维。
