Eade Colleen R, Diaz Camila, Chen Sixue, Cole Amy L, Cole Alexander M
4000 Central Florida Blvd, Building 20, Room 236, University of Central Florida, Orlando, FL 32816, USA.
Protein Pept Lett. 2015;22(8):672-80. doi: 10.2174/0929866522666150309155735.
Bacterial vaginosis is a common reproductive infection in which commensal vaginal lactobacilli are displaced by a mixed population of pathogenic bacteria. Bacterial vaginosis increases susceptibility to HIV, and it has been suggested that host innate immune responses to pathogenic bacteria contribute to enhanced infection, yet the cellular mechanisms mediating the increased HIV susceptibility remain uncharacterized. We evaluated the HIV-enhancing effects of bacterial vaginosis by inoculating endocervical epithelia with Atopobium vaginae, a bacterial vaginosis-associated bacteria, and assaying secreted factors for HIV-enhancing activity. When epithelia and A. vaginae were cocultured, we observed increased HIV-enhancing activity mediated by secreted low molecular weight factors. From this complex mixture we identified several upregulated host proteins, which functioned in combination to enhance HIV infection. These studies suggest that the host immune response to bacterial vaginosis-associated bacteria results in the release of HIV-enhancing factors. The combined activity of bacterial vaginosis-induced proteins likely mediates HIV enhancement.
细菌性阴道病是一种常见的生殖系统感染，其中阴道内的共生乳酸杆菌被一群致病细菌所取代。细菌性阴道病会增加感染HIV的易感性，有人认为宿主对致病细菌的固有免疫反应会导致感染增强，然而，介导HIV易感性增加的细胞机制仍未明确。我们通过用阴道阿托波菌（一种与细菌性阴道病相关的细菌）接种宫颈上皮，并检测分泌因子的HIV增强活性，来评估细菌性阴道病对HIV感染的增强作用。当上皮细胞与阴道阿托波菌共培养时，我们观察到由分泌的低分子量因子介导的HIV增强活性增加。从这个复杂的混合物中，我们鉴定出了几种上调的宿主蛋白，它们共同作用以增强HIV感染。这些研究表明，宿主对与细菌性阴道病相关细菌的免疫反应导致了HIV增强因子的释放。细菌性阴道病诱导蛋白的联合活性可能介导了HIV感染的增强。
