Sándor Gábor L, Kiss Zoltán, Bocskai Zoltán I, Kolev Krasimir, Takács Ágnes I, Juhász Éva, Kránitz Kinga, Tóth Gábor, Gyenes Andrea, Bojtár Imre, Juhász Tibor, Nagy Zoltán Z
J Refract Surg. 2015 Mar;31(3):153-7. doi: 10.3928/1081597X-20150220-02.
To evaluate and compare the mechanical properties of anterior capsule opening performed with femtosecond laser capsulotomy at different energy settings in ex vivo porcine anterior lens capsule specimens.
Twenty-five fresh porcine eyes per group were included in the study. Femtosecond laser capsulotomy was performed with three different pulse energy levels: 2 µJ (low energy group), 5 µJ (intermediate energy group), and 10 µJ (high energy group). The capsule openings were stretched with universal testing equipment until they ruptured. The morphologic profile of the cut capsule edges was evaluated using scanning electron microscopy.
The high energy group had significantly lower rupture force (108 ± 14 mN) compared to the intermediate energy group (118 ± 10 mN) (P < .05) and low energy group (119 ± 11 mN) (P < .05), but the difference between the intermediate energy and low energy groups was not significant (P = .9479). The high energy group had significantly lower circumference stretching ratio (144% ± 3%) compared to the intermediate energy group (148% ± 3%) (P < .05) and low energy group (148% ± 3%) (P < .05), but the difference between the intermediate energy group and low energy group was not significant (P = .9985). Scanning electron microscopy images showed that the edge was only serrated with low and intermediate energy, but additional signs of collagen melting and denaturation were observed at high energy.
Anterior capsule openings created at a high energy level were slightly weaker and less extensible than those created at low or intermediate levels, possibly due to the increased thermal effect of photo-disruption.
评估并比较在离体猪前囊膜标本中，不同能量设置的飞秒激光截囊术所形成的前囊膜开口的机械性能。
每组纳入25只新鲜猪眼进行研究。使用三种不同的脉冲能量水平进行飞秒激光截囊术：2微焦（低能量组）、5微焦（中能量组）和10微焦（高能量组）。使用通用测试设备拉伸囊膜开口直至破裂。使用扫描电子显微镜评估切开的囊膜边缘的形态轮廓。
与中能量组（118±10毫牛）（P<.05）和低能量组（119±11毫牛）（P<.05）相比，高能量组的破裂力显著更低（108±14毫牛），但中能量组和低能量组之间的差异不显著（P=.9479）。与中能量组（148%±3%）（P<.05）和低能量组（148%±3%）（P<.05）相比，高能量组的周长拉伸率显著更低（144%±3%），但中能量组和低能量组之间的差异不显著（P=.9985）。扫描电子显微镜图像显示，低能量和中能量时边缘仅呈锯齿状，但在高能量时观察到胶原熔化和变性的额外迹象。
高能量水平下形成的前囊膜开口比低能量或中能量水平下形成的开口稍弱且伸展性稍差，这可能是由于光破碎的热效应增加所致。
