Zhu Kun-Jie, Sun Jian-Ning
Zhongguo Zhong Yao Za Zhi. 2014 Oct;39(20):4007-12.
To explore the effect and mechanism of cinnabaris and realgar in promoting awake effect of endotoxin- induced brain injury rat applied with Angong Niuhuang Wan.
Normal rats implanted cortical electrode in advance were divided into 6 groups: control, model, the Angong Niuhuang Wan (AGNH, 0.4, 0.2 g · kg(-1)), the Angong Niuhuang Wan without cinnabaris and realgar (QZX-AGNH, 0.32, 0.16 g · kg(-1)). Rats in the control and model groups were given distilled water. After three days of intragastric administration, the brain injury model was injected with endotoxin through tail vein. Then trace electro-corticogram (EcoG) 1-6 h after LPS injection, and compare the power and relative power of beta (β) and delta-waves (δ) at 6 h of these groups. The content of acetylcholine (Ach) and the affinity of M-receptor (M-R) in cortex and brainstem were detected by alkaline hydroxylamine colorimetric method and radioactive ligand binding assay, respectively.
AGNH (0.4, 0.2 g · kg(-1)) could increase the power and relative power of β and AGNH (0.4 g · kg(-1)) showed better action on brain electrical activation. QZX-AGNH showed weak effect on it. AGNH (0.4 g · kg(-1)) could increase the affinity of M-R in cortex and the content of Ach in brainstem. The action of QZX-AGNH was not obvious.
In endotoxin-induced brain injury rats, AGNH can raise the cholinergic system function of cortex, and strengthen the uplink of cortex activation of brainstem cholinergic system, improve the level of cortical activity and enhance the activation of EcoG to promote the body's awakening. QZX-AGNH show weak effect. Cinnabaris and realgar play an important role in promoting awake effect in endotoxin-induced brain injury applied with Angong Niuhuang Wan. The mechanism may be related to cortical and brainstem cholinergic system function.
探讨朱砂、雄黄对安宫牛黄丸改善内毒素致脑损伤大鼠觉醒作用的影响及其机制。
将预先植入皮层电极的正常大鼠分为6组：对照组、模型组、安宫牛黄丸组（0.4、0.2 g·kg⁻¹）、去朱砂雄黄安宫牛黄丸组（0.32、0.16 g·kg⁻¹）。对照组和模型组大鼠给予蒸馏水。连续灌胃3天后，模型组大鼠经尾静脉注射内毒素建立脑损伤模型。注射脂多糖后1 - 6小时记录微量脑电图（EcoG），比较各组6小时时β波和δ波的功率及相对功率。分别采用碱性羟胺比色法和放射性配体结合法检测皮层和脑干中乙酰胆碱（Ach）含量及M受体（M-R）亲和力。
安宫牛黄丸（0.4、0.2 g·kg⁻¹）可提高β波功率及相对功率，安宫牛黄丸（0.4 g·kg⁻¹）对脑电激活作用更佳。去朱砂雄黄安宫牛黄丸作用较弱。安宫牛黄丸（0.4 g·kg⁻¹）可提高皮层M-R亲和力及脑干Ach含量。去朱砂雄黄安宫牛黄丸作用不明显。
在内毒素致脑损伤大鼠中，安宫牛黄丸可提高皮层胆碱能系统功能，增强脑干胆碱能系统对皮层激活的上行作用，改善皮层活动水平，增强EcoG激活以促进机体觉醒。去朱砂雄黄安宫牛黄丸作用较弱。朱砂、雄黄在安宫牛黄丸改善内毒素致脑损伤大鼠觉醒作用中起重要作用。其机制可能与皮层和脑干胆碱能系统功能有关。
