[炭疽芽孢杆菌中的成簇规律间隔短回文重复序列（CRISPR）位点]

[Clustered regularly interspaced short palindromic repeats (CRISPR) site in Bacillus anthracis].

作者信息

Gao Zhiqi, Wang Dongshu, Feng Erling, Wang Bingxiang, Hui Yiming, Han Shaobo, Jiao Lei, Liu Xiankai, Wang Hengliang

出版信息

Wei Sheng Wu Xue Bao. 2014 Nov 4;54(11):1362-8.

Abstract

OBJECTIVE

To investigate the polymorphism of clustered regularly interspaced short palindromic repeats (CRISPR) in Bacillu santhracis and the application to molecular typing based on the polymorphism of CRISPR in B. anthracis.

METHODS

We downloaded the whole genome sequence of 6 B. anthracis strains and extracted the CRISPR sites. We designed the primers of CRISPR sites and amplified the CRISPR fragments in 193 B. anthracis strains by PCR and sequenced these fragments. In order to reveal the polymorphism of CRISPR in B. anthracis, wealigned all the extracted sequences and sequenced results by local blasting. At the same time, we also analyzed the CRISPR sites in B. cereus and B. thuringiensis.

RESULTS

We did not find any polymorphism of CRISPR in B. anthracis.

CONCLUSION

The molecular typing approach based on CRISPR polymorphism is not suitable for B. anthracis, but it is possible for us to distinguish B. anthracis from B. cereus and B. thuringiensis.

摘要

目的

研究炭疽芽孢杆菌中规律成簇间隔短回文重复序列（CRISPR）的多态性及其基于该多态性在分子分型中的应用。

方法

下载6株炭疽芽孢杆菌的全基因组序列，提取CRISPR位点。设计CRISPR位点引物，通过PCR扩增193株炭疽芽孢杆菌中的CRISPR片段并进行测序。为揭示炭疽芽孢杆菌中CRISPR的多态性，通过局部比对将所有提取的序列与测序结果进行比对。同时，也分析了蜡样芽孢杆菌和苏云金芽孢杆菌中的CRISPR位点。