Badgujar Prarabdh C, Pawar Nitin N, Chandratre Gauri A, Telang A G, Sharma A K
Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar 243 122, India.
Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar 243 122, India.
Pestic Biochem Physiol. 2015 Feb;118:10-8. doi: 10.1016/j.pestbp.2014.10.013. Epub 2014 Oct 28.
Fipronil is a relatively new insecticide of the phenpyrazole group. Fipronil-induced effects on antioxidant system and oxidative stress biomarkers are yet to be studied in vivo. The present study was undertaken to evaluate fipronil-induced alterations in the blood biochemical markers and tissue antioxidant enzymes after oral exposure in mice and to explore possible protective effect of pre-treatment of antioxidant vitamins against these alterations. Mice were divided into eight groups containing control, test and amelioration groups. Mice in the test groups were exposed to different doses of fipronil, i.e., 2.5, 5 and 10 mg/kg bw, respectively for 28 days. Mice in the amelioration groups were treated with vitamin E or vitamin C (each at 100 mg/kg) 2 h prior to high dose (10 mg/kg) of fipronil. Fipronil exposure at three doses caused significant increase in the blood biochemical markers, lipid peroxidation and prominent histopathological alterations; while level of antioxidant enzymes was severely decreased both in kidney and brain tissues. Prior administration of vitamin E or vitamin C in the fipronil exposed mice led to decrease in lipid peroxidation and significant increase in activities of antioxidants, viz., glutathione, total thiol, superoxide dismutase and catalase. Vitamin E and vitamin C administration in fipronil exposed mice also improved histological architecture of the kidney and brain when compared with fipronil alone treated groups. Thus, results of the present study demonstrated that in vivo fipronil exposure induces oxidative stress and pre-treatment with vitamin E or C can protect mice against this oxidative insult.
氟虫腈是一种相对较新的吡唑类杀虫剂。氟虫腈对抗氧化系统和氧化应激生物标志物的影响尚未在体内进行研究。本研究旨在评估小鼠经口暴露后氟虫腈引起的血液生化标志物和组织抗氧化酶的变化，并探讨抗氧化维生素预处理对这些变化的可能保护作用。将小鼠分为八组，包括对照组、试验组和改善组。试验组小鼠分别暴露于不同剂量的氟虫腈，即2.5、5和10mg/kg体重，持续28天。改善组小鼠在高剂量（10mg/kg）氟虫腈暴露前2小时用维生素E或维生素C（各100mg/kg）处理。三个剂量的氟虫腈暴露导致血液生化标志物、脂质过氧化显著增加以及明显的组织病理学改变；而肾脏和脑组织中的抗氧化酶水平均严重降低。在暴露于氟虫腈的小鼠中预先给予维生素E或维生素C可导致脂质过氧化减少，抗氧化剂（即谷胱甘肽、总硫醇、超氧化物歧化酶和过氧化氢酶）的活性显著增加。与仅用氟虫腈处理的组相比，在暴露于氟虫腈的小鼠中给予维生素E和维生素C也改善了肾脏和脑的组织结构。因此，本研究结果表明，体内氟虫腈暴露会诱导氧化应激，而用维生素E或C预处理可保护小鼠免受这种氧化损伤。
