Ghazanfar Madiha, Shahid Sana, Qureshi Irfan Zia
Laboratory of Animal and Human Physiology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320 Pakistan.
Laboratory of Animal and Human Physiology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320 Pakistan.
Aquat Toxicol. 2018 Mar;196:43-52. doi: 10.1016/j.aquatox.2017.12.015. Epub 2018 Jan 5.
In the present study, potential protective role of Vitamin C (l-ascorbic acid) was investigated in aquaria acclimated common carp (Cyprinus carpio) following exposure for 96 h to combined toxic doses of fipronil (FP) and buprofezin (BPFN) insecticides in combination (FP: 200 μg/L; 4.57 × 10 mol/L and BPFN: 50 mg/L; 1.64 × 10 mol/L). At end of 96 h exposure, fish were supplemented with low (25 mg/L) and high (50 mg/L) doses of Vitamin C, added once daily to aquaria water for continuous three weeks. Appropriate control groups were run in parallel. Fish behavior was monitored throughout for signs of toxicity. At completion of experiments, liver, kidney, brain and gills were excised for toxicity assessment and possible remediation by the Vitamin C through biochemical determination of reactive oxygen species (ROS), thiobarbituric acid reactive substances or TBARS, reduced glutathione (GSH) and total protein content, levels of catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD), and the Comet assay. Hepatosomatic index (HSI), condition factor (CF), survival rate (SR), and combination index (CI) were also determined. Data were compared statistically at p < 0.05. Results showed significant behavioral and biochemical alterations, and DNA damage in the fish group exposed to FP and BPFN in combination. In fish groups supplemented with Vitamin C following FP and BPFN treatment, significant alleviation in tissue damage and toxic effects was represented by substantial decreases in ROS and TBARS production (p < 0.001), along with a concomitant significant increase in the survival rate, GSH and total protein content, HSI, CF, and activities of SOD, CAT and POD enzymes (p < 0.001). Mean tail length of comet and percent tail DNA decreased significantly (p < 0.001), which indicated amelioration of DNA damage. The study concludes that Vitamin C is an effective remedial treatment against FP and BPFN-induced damage in exposed fish.
在本研究中，调查了维生素C（L-抗坏血酸）对水族箱中适应性养殖的鲤鱼（Cyprinus carpio）的潜在保护作用。这些鲤鱼在96小时内暴露于氟虫腈（FP）和噻嗪酮（BPFN）两种杀虫剂的联合毒性剂量下（FP：200μg/L；4.57×10⁻⁴mol/L，BPFN：50mg/L；1.64×10⁻³mol/L）。在96小时暴露结束时，给鱼补充低剂量（25mg/L）和高剂量（50mg/L）的维生素C，每天向水族箱水中添加一次，持续三周。同时设置了适当的对照组。在整个过程中监测鱼的行为以观察毒性迹象。实验结束时，切除肝脏、肾脏、大脑和鳃用于毒性评估，并通过生化测定活性氧（ROS）、硫代巴比妥酸反应性物质（TBARS）、还原型谷胱甘肽（GSH）和总蛋白含量、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和过氧化物酶（POD）的水平以及彗星试验，来评估维生素C可能的修复作用。还测定了肝体指数（HSI）、条件因子（CF）、存活率（SR）和联合指数（CI）。数据在p<0.05水平进行统计学比较。结果显示，联合暴露于FP和BPFN的鱼组出现了显著的行为和生化改变以及DNA损伤。在FP和BPFN处理后补充维生素C的鱼组中，组织损伤和毒性作用得到显著缓解，表现为ROS和TBARS生成量大幅下降（p<0.001），同时存活率、GSH和总蛋白含量、HSI、CF以及SOD、CAT和POD酶的活性显著增加（p<0.001）。彗星的平均尾长和尾DNA百分比显著下降（p<0.001），这表明DNA损伤得到改善。该研究得出结论，维生素C是一种有效治疗暴露鱼类中FP和BPFN诱导损伤的补救方法。
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