在二氧化碳浓度升高条件下，三唑磷施用于转基因Bt水稻对褐飞虱营养指标、Nlvg表达及种群增长的影响。
The effects of triazophos applied to transgenic Bt rice on the nutritional indexes, Nlvg expression, and population growth of Nilaparvata lugens Stål under elevated CO₂.
作者信息
Ge Lin-Quan, Sun Yu-Cheng, Ouyang Fang, Wu Jin-Cai, Ge Feng
机构信息
State Key Laboratory of Integrated Management of Pest and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
State Key Laboratory of Integrated Management of Pest and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
出版信息
Pestic Biochem Physiol. 2015 Feb;118:50-7. doi: 10.1016/j.pestbp.2014.11.010. Epub 2014 Dec 2.
The brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is a typical pest in which population resurgence can be induced by insecticides. Warmer global temperatures, associated with anthropogenic climate change, are likely to have marked ecological effects on terrestrial ecosystems. However, the effects of elevated CO2 (eCO2) concentrations on the resurgence of N. lugens that have been treated with pesticides used for transgenic Bt rice cultivation are not fully understood. The present study investigated changes in the protein content, soluble sugar content, free amino acid level, vitellogenin (Nlvg) mRNA expression, and the population growth of N. lugens on transgenic Bt rice (TT51) following triazaophos foliar spray under conditions of eCO2. The results showed that the protein content in the fat bodies and ovaries of N. lugens adult females in TT51 treated with 40 ppm triazophos under eCO2 was significantly higher than under ambient CO2 (aCO2) and was also higher than that in females feeding on the non-transgenic parent (MH63) under aCO2 at different days after emergence (DAEs). The soluble sugar content and free amino level of adult females in TT51 treated with 40 ppm triazophos under eCO2 was significantly higher than under aCO2 and was also higher than in MH63 under aCO2 at 1 and 3 DAE. The Nlvg mRNA expression level of N. lugens adult females in TT51 treated with 40 ppm triazophos under eCO2 was significantly higher than under aCO2 and was also higher than in MH63 under aCO2 at 1 and 3 DAE. The population number of N. lugens in TT51 treated with 40 ppm triazophos under eCO2 was significantly higher than under aCO2 and was also higher than in MH63 under aCO2. The present findings provide important information for integrated pest management with transgenic varieties and a better understanding of the resurgence mechanism of N. lugens under eCO2.
褐飞虱，Nilaparvata lugens (Stål)（半翅目：飞虱科），是一种典型的害虫，其种群数量会因杀虫剂而回升。与人为气候变化相关的全球气温升高，可能会对陆地生态系统产生显著的生态影响。然而，二氧化碳浓度升高（eCO2）对经用于转基因Bt水稻种植的农药处理过的褐飞虱种群回升的影响尚未完全明确。本研究调查了在eCO2条件下，经三唑磷叶面喷施后，转基因Bt水稻（TT51）上褐飞虱的蛋白质含量、可溶性糖含量、游离氨基酸水平、卵黄原蛋白（Nlvg）mRNA表达以及种群增长的变化。结果表明，在eCO2条件下，用40 ppm三唑磷处理的TT51上褐飞虱成年雌虫脂肪体和卵巢中的蛋白质含量，显著高于环境二氧化碳（aCO2）条件下，且在羽化后不同天数（DAEs）也高于aCO2条件下取食非转基因亲本（MH63）的雌虫。在eCO2条件下，用40 ppm三唑磷处理的TT51上成年雌虫的可溶性糖含量和游离氨基酸水平，显著高于aCO2条件下，且在羽化后1天和3天也高于aCO2条件下的MH63。在eCO2条件下，用40 ppm三唑磷处理的TT51上褐飞虱成年雌虫的Nlvg mRNA表达水平，显著高于aCO2条件下，且在羽化后1天和3天也高于aCO2条件下的MH63。在eCO2条件下，用40 ppm三唑磷处理的TT51上褐飞虱的种群数量，显著高于aCO2条件下，且也高于aCO2条件下的MH63。本研究结果为转基因品种的害虫综合管理以及更好地理解eCO2条件下褐飞虱的回升机制提供了重要信息。
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