Erasmus Daniel J, Brewer Sharon E, Cinel Bruno
Biochemistry and Molecular Biology Program, University of Northern British Columbia, Prince George, British Columbia, V2N 4Z9, Canada.
Biochem Mol Biol Educ. 2015 Mar-Apr;43(2):121-5. doi: 10.1002/bmb.20865. Epub 2015 Mar 6.
Undergraduate laboratories expose students to a wide variety of topics and techniques in a limited amount of time. This can be a challenge and lead to less exposure to concepts and activities in bio-inorganic chemistry and analytical chemistry that are closely-related to biochemistry. To address this, we incorporated a new iron determination by atomic absorption spectroscopy exercise as part of a five-week long laboratory-based project on the purification of myoglobin from beef. Students were required to prepare samples for chemical analysis, operate an atomic absorption spectrophotometer, critically evaluate their iron data, and integrate these data into a study of myoglobin.
本科实验室在有限的时间内让学生接触到各种各样的主题和技术。这可能是一项挑战，导致学生较少接触到与生物化学密切相关的生物无机化学和分析化学中的概念和活动。为了解决这个问题，我们将一项通过原子吸收光谱法测定铁的新实验纳入了一个为期五周的基于实验室的项目，该项目是关于从牛肉中纯化肌红蛋白的。学生们需要准备化学分析样品、操作原子吸收分光光度计、严格评估他们的铁数据，并将这些数据整合到对肌红蛋白的研究中。
