Olenik C, Meyer D K, Marksteiner J, Sperk G
Department of Pharmacology, University of Freiburg, Republic of Germany.
Synapse. 1989;4(3):223-8. doi: 10.1002/syn.890040307.
Concentrations of preprosomatostatin-mRNA and preprocholecystokinin-mRNA were determined by Northern blot analysis in rats 2, 10, and 30 days after strong seizures induced by a single intraperitoneal injection of kainic acid. At all time intervals examined, levels of preprosomatostatin-mRNA were increased in the frontal cortex; so were levels of preprocholecystokinin-mRNA in the striatum. Transient increases, i.e., 2 days after kainic acid, of preprocholecystokinin-mRNA were observed in the frontal cortex and the substantia nigra. Preprocholecystokinin-mRNA was reduced in the hippocampus 2 and 10 days after kainic acid. Both preprosomatostatin- and preprocholecystokinin-mRNA levels showed a tendency to be reduced in the amygdala/pyriform cortex at all three time intervals. The increases in mRNA levels suggest enhanced rates of synthesis of the respective neuropeptides subsequent to kainic acid-induced seizures. They may also reflect a prolonged increase in the activity of the respective peptide-containing neurons. This is of special interest in the frontal cortex, since in this area both neuropeptides are found in interneurons and are widely colocalized with gamma-aminobutyric acid.
通过Northern印迹分析测定了单次腹腔注射海藻酸诱导强烈癫痫发作后2天、10天和30天大鼠前促生长抑素mRNA和前促胆囊收缩素mRNA的浓度。在所有检测的时间间隔内，额叶皮质中前促生长抑素mRNA的水平均升高；纹状体中前促胆囊收缩素mRNA的水平也升高。在额叶皮质和黑质中观察到，海藻酸注射后2天，前促胆囊收缩素mRNA短暂升高。海藻酸注射后2天和10天，海马体中前促胆囊收缩素mRNA减少。在所有三个时间间隔内，杏仁核/梨状皮质中前促生长抑素和前促胆囊收缩素mRNA水平均有降低趋势。mRNA水平的升高表明，海藻酸诱导癫痫发作后，相应神经肽的合成速率增强。它们也可能反映了含相应肽的神经元活动的长期增加。这在额叶皮质中尤为有趣，因为在该区域，两种神经肽都存在于中间神经元中，并与γ-氨基丁酸广泛共定位。
