Dhiman Alisha, Gopalani Monisha, Bhatnagar Rakesh
Laboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India.
Laboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India.
Biochem Biophys Res Commun. 2015 Apr 17;459(4):623-8. doi: 10.1016/j.bbrc.2015.02.159. Epub 2015 Mar 6.
WalRK Two Component System (TCS) of Bacillus anthracis forms a functional TCS. This report elaborates upon the WalRK genomic architecture, promoter structure, promoter activity and expression under various stress conditions in B. anthracis. 5' RACE located the WalRK functional promoter within 317 bp region upstream of WalR. Reporter gene assays demonstrated maximal promoter activity during early growth phases indicating utility in exponential stages of growth. qRT-PCR showed upregulation of WalRK transcripts during temperature and antibiotic stress. However, WalR overexpression did not affect the tested antibiotic MIC values in B. anthracis. Collectively, these results confirm that WalRK responds to cell envelope stress in B. anthracis.
炭疽芽孢杆菌的WalRK双组分系统（TCS）构成了一个功能性TCS。本报告详细阐述了炭疽芽孢杆菌中WalRK的基因组结构、启动子结构、启动子活性以及在各种应激条件下的表达情况。5' RACE将WalRK功能性启动子定位在WalR上游317 bp区域内。报告基因检测表明，在生长早期阶段启动子活性最高，这表明其在指数生长期具有实用性。qRT-PCR显示，在温度和抗生素应激期间WalRK转录本上调。然而，WalR的过表达并未影响炭疽芽孢杆菌中测试抗生素的最低抑菌浓度（MIC）值。总体而言，这些结果证实了WalRK在炭疽芽孢杆菌中对细胞壁应激有反应。
