Rasmussen H B, Brandrup F, Andersen J, Hagdrup H
Department of Dermatology, Odense University Hospital, Denmark.
Acta Derm Venereol. 1989;69(6):515-9.
The sensitivity of the indirect immunofluorescence (IIF) technique for detection of circulating basement membrane zone (BMZ) antibodies was evaluated, employing NaCl-separated human skin and intact skin as substrate. Consecutive serum samples from 12 patients with clinically, histologically and immunohistologically verified bullous pemphigoid (BP) were investigated in parallel on both substrates, in dilutions ranging from 1:10 to 1:1,280. All BP sera showed linear deposits of IgG at the BMZ on intact skin, with titres ranging from 10 to 160. On NaCl-separated skin, all BP sera produced a linear epidermal fluorescent band for IgG, with titres ranging from 80 to 1,280. None of the sera showed deposits of IgM anti-BMZ antibodies. Sera from 5 healthy donors (dilutions 1:10) produced no fluorescence, either on intact or on NaCl-separated skin. The serum-titres of circulating anti-BMZ IgG antibodies in 2 patients with corticosteroid-resistant BP were significantly reduced (from 160 to less than 10) during treatment with plasmapheresis, when using NaCl-separated skin as substrate for IIF, whereas the serum-titres showed insignificant reduction (from 20 to less than 10), when using intact skin as substrate. We conclude that the IIF method is more sensitive for detection of circulating anti-BMZ antibodies, when NaCl-separated skin as compared with intact human skin is employed as substrate.
采用氯化钠分离的人皮肤和完整皮肤作为底物，评估间接免疫荧光（IIF）技术检测循环基底膜带（BMZ）抗体的敏感性。对12例经临床、组织学和免疫组织学证实为大疱性类天疱疮（BP）患者的连续血清样本，在两种底物上以1:10至1:1280的稀释度进行平行研究。所有BP血清在完整皮肤上的BMZ处均显示IgG线性沉积，滴度范围为10至160。在氯化钠分离的皮肤上，所有BP血清均产生IgG的线性表皮荧光带，滴度范围为80至1280。无一血清显示IgM抗BMZ抗体沉积。5名健康供者的血清（稀释度1:10）在完整皮肤或氯化钠分离的皮肤上均未产生荧光。在以氯化钠分离的皮肤作为IIF底物时，2例对皮质类固醇耐药的BP患者在进行血浆置换治疗期间，循环抗BMZ IgG抗体的血清滴度显著降低（从160降至低于10），而以完整皮肤作为底物时，血清滴度降低不显著（从20降至低于10）。我们得出结论，与完整的人皮肤相比，当以氯化钠分离的皮肤作为底物时，IIF方法检测循环抗BMZ抗体更敏感。
