Nurhasan M, Roos N, Aristizabal Henao J J, Chamnan C, Stark K D, Lauritzen L
Department of Nutrition, Exercise and Sports, University of Copenhagen, Denmark.
Department of Kinesiology, University of Waterloo, Canada.
Prostaglandins Leukot Essent Fatty Acids. 2015 May;96:57-61. doi: 10.1016/j.plefa.2015.02.001. Epub 2015 Feb 14.
Fatty acid analysis requires standardized collection and storage of samples, which can be a challenge under field conditions. This study describes the effect of storage temperature on fatty acid composition in two sets of whole blood samples collected from 66 children in a rural area in Cambodia. The samples were stored with butylated hydroxytoluene at -20 °C and -80 °C and the latter required extra transfers due to storage facility limitation. Fatty acid composition was analyzed by high-throughput gas-chromatography and evaluated by paired t-tests and Bland-Altman plots. Total amounts of fat in -20 °C and -80 °C samples did not differ, but there was relatively more highly unsaturated fatty acids (15.8 ± 2.7 vs. 14.4 ± 2.5%, p < 0.001) and a lower n-6/n-3 ratio (6.4 ± 1.4 vs. 6.9 ± 1.4, p < 0.001) in the -20 °C samples. Our results indicate that the importance of storage temperature should be evaluated in the context of storage facility availability and risk of temperature fluctuations during transport.
脂肪酸分析需要对样本进行标准化的采集和储存，而这在野外条件下可能是一项挑战。本研究描述了储存温度对从柬埔寨农村地区66名儿童采集的两组全血样本中脂肪酸组成的影响。样本在-20°C和-80°C下与丁基羟基甲苯一起储存，由于储存设施的限制，后者需要额外转移。通过高通量气相色谱法分析脂肪酸组成，并通过配对t检验和布兰德-奥特曼图进行评估。-20°C和-80°C样本中的总脂肪量没有差异，但-20°C样本中高度不饱和脂肪酸相对较多（15.8±2.7%对14.4±2.5%，p<0.001），n-6/n-3比值较低（6.4±1.4对6.9±1.4，p<0.001）。我们的结果表明，应根据储存设施的可用性以及运输过程中温度波动的风险来评估储存温度的重要性。
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