Pupillo Daniele, Simonato Manuela, Cogo Paola E, Lapillonne Alexandre, Carnielli Virgilio P
Division of Neonatology, Polytechnic University of Marche and Salesi's Children Hospital, Via Corridoni 11, 62123, Ancona, Italy.
Pediatric Research Institute "Città della Speranza", Padua, Italy.
Lipids. 2016 Feb;51(2):193-8. doi: 10.1007/s11745-015-4111-z. Epub 2016 Jan 9.
Finger or heel-pricked blood sampling for fatty acid analysis is suitable especially in newborn infants where blood sampling is difficult and phlebotomy for research can be unethical. The aim of this study was to evaluate dried blood long chain polyunsaturated fatty acids (LC-PUFA) stability during storage at -28 °C. We collected 12 blood cord samples that were analyzed immediately after blood drawing, with and without drying the blood on filter paper. Dried samples were then analyzed 7 days and 1, 3, and 6 months after collection. Butylated hydroxytoluene was added to all samples. Fatty acid composition and (13)C enrichment were measured by gas chromatography and by gas chromatography-isotope ratio mass spectrometry, respectively. The fatty acid composition, expressed in mol%, of the major LC-PUFA at day 7 was not statistically different from time 0, however lower values were found by the first month of storage. The (13)C enrichment of 20:4n-6 and 22:6n-3 did not differ during the whole study period. LC-PUFA analysis from dried umbilical cord blood in neonates should be performed within a week, major losses of LC-PUFA occur afterwards. However, fatty acids obtained from dried blood maintain their (13)C enrichment value for up to 6 months and thus these samples are suitable for natural abundance isotopic studies.
通过手指或足跟采血进行脂肪酸分析特别适用于新生儿，因为新生儿采血困难，且为研究进行静脉穿刺可能不符合伦理规范。本研究的目的是评估干血中长链多不饱和脂肪酸（LC-PUFA）在-28°C储存期间的稳定性。我们收集了12份脐带血样本，在采血后立即进行分析，包括不将血液干燥在滤纸上和干燥在滤纸上两种情况。然后在采集后7天、1个月、3个月和6个月对干燥后的样本进行分析。所有样本均添加了丁基羟基甲苯。分别通过气相色谱法和气相色谱-同位素比率质谱法测量脂肪酸组成和（13）C富集情况。以mol%表示的主要LC-PUFA在第7天的脂肪酸组成与0时刻无统计学差异，但在储存第一个月时发现值较低。在整个研究期间，20:4n-6和22:6n-3的（13）C富集情况没有差异。新生儿干脐带血的LC-PUFA分析应在一周内进行，此后LC-PUFA会出现大量损失。然而，从干血中获得的脂肪酸在长达6个月的时间内保持其（13）C富集值，因此这些样本适用于天然丰度同位素研究。
