Holmes R P, Craddock G, Espeland M A, Assimos D G, Dean R H
Urology Section, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27103.
Clin Chim Acta. 1989 Dec 29;186(1):1-9. doi: 10.1016/0009-8981(89)90196-4.
The urinary secretion of two lysosomal enzymes, N-acetyl-D-glucosaminidase (NAG, EC 3.2.1.30) and beta-glucuronidase (GLR, EC 3.2.1.31), and two brush border enzymes, alanine aminopeptidase (AAP, EC 3.4.11.2) and gamma-glutamyltransferase (GGT, EC 2.3.2.2), was examined in apparently healthy individuals and in patients before and after renovascular surgery for treatment of hypertension. Eight out of nine patients had elevated levels of at least one enzyme before surgery. The ranking in their frequency of elevation was NAG greater than AAP greater than GLR greater than GGT. In comparing the release of any two enzymes in apparently healthy individuals, the release was coordinated except for GGT and GLR. In individual patients following surgery the excretion of the lysosomal enzymes was highly coordinated whereas the release of the brush border enzymes was less coordinated. Comparisons of lysosomal to brush border enzyme activities revealed dissimilar release patterns between these two classes of enzymes. Analysis of variance over the entire hospitalization period showed that NAG/GLR (p = 0.42) and AAP/GGT (p = 0.12) did not vary significantly whereas all comparisons of lysosomal to brush border enzymes varied significantly (p less than or equal to 0.03). These results indicate that enzymes derived from different subcellular organelles, lysosomes or brush borders, have similar release patterns. However, the lack of a significant correlation between lysosomal and brush border enzyme excretion implies that the two processes are not interdependent. These studies further suggest that the transient pathophysiological changes that occur within renal cells following renovascular surgery affect these cellular components in different ways.
在看似健康的个体以及接受肾血管手术治疗高血压的患者术前和术后，检测了两种溶酶体酶，即N - 乙酰 - D - 氨基葡萄糖苷酶（NAG，EC 3.2.1.30）和β - 葡萄糖醛酸酶（GLR，EC 3.2.1.31），以及两种刷状缘酶，即丙氨酸氨基肽酶（AAP，EC 3.4.11.2）和γ - 谷氨酰转移酶（GGT，EC 2.3.2.2）的尿液分泌情况。9名患者中有8名在手术前至少有一种酶水平升高。它们升高频率的排序为NAG＞AAP＞GLR＞GGT。在比较看似健康个体中任意两种酶的释放情况时，除了GGT和GLR外，释放是协同的。在术后的个体患者中，溶酶体酶的排泄高度协同，而刷状缘酶的释放协同性较差。溶酶体酶与刷状缘酶活性的比较显示这两类酶之间存在不同的释放模式。对整个住院期间的方差分析表明，NAG/GLR（p = 0.42）和AAP/GGT（p = 0.12）没有显著变化，而溶酶体酶与刷状缘酶的所有比较均有显著变化（p≤0.03）。这些结果表明，源自不同亚细胞器（溶酶体或刷状缘）的酶具有相似的释放模式。然而，溶酶体酶与刷状缘酶排泄之间缺乏显著相关性意味着这两个过程并非相互依存。这些研究进一步表明，肾血管手术后肾细胞内发生的短暂病理生理变化以不同方式影响这些细胞成分。
