Zhang Bin, Yu Lei, Zhang Ruijing, Liu Yang, Liu Rutao
School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, 27# Shanda South Road, Jinan, 250100, People's Republic of China.
College of Population Resources and Environment, Shandong Normal University, Jinan, 250014, People's Republic of China.
Luminescence. 2015 Dec;30(8):1195-200. doi: 10.1002/bio.2880. Epub 2015 Mar 8.
Silver nanoparticles (nanoAg) are used more and more widely, particularly because of their antimicrobial properties. The effect of exposure to nanoAg on the structure of superoxide dismutase (SOD) was thoroughly investigated using fluorescence measurements, synchronous fluorescence spectroscopy, steady-state and time-resolved fluorescence quenching measurements, UV/Vis absorption spectroscopy, resonance light scattering (RLS), circular dichroism (CD), isothermal titration calorimetry (ITC) and high-resolution transmission electron microscopy (HRTEM). Through van der Waal's force, nanoAg interacted with Cu-Zn SOD and influenced the active site by inducing structural changes, which influenced the function of SOD. The fluorescence studies show that both static and dynamic quenching processes occur. This paper provides reference data for toxicological studies of nanoAg, which are important in the future development of nanotechnology.
银纳米颗粒（纳米银）的应用越来越广泛，特别是因其抗菌特性。通过荧光测量、同步荧光光谱、稳态和时间分辨荧光猝灭测量、紫外/可见吸收光谱、共振光散射（RLS）、圆二色性（CD）、等温滴定量热法（ITC）和高分辨率透射电子显微镜（HRTEM），深入研究了暴露于纳米银对超氧化物歧化酶（SOD）结构的影响。纳米银通过范德华力与铜锌超氧化物歧化酶相互作用，并通过诱导结构变化影响活性位点，进而影响超氧化物歧化酶的功能。荧光研究表明，静态和动态猝灭过程均会发生。本文为纳米银的毒理学研究提供了参考数据，这对纳米技术的未来发展具有重要意义。
