Kumar Amit, Sharma Arun, Duseja Ajay, Das Ashim, Dhiman Radha K, Chawla Yogesh K, Kohli Krishan K, Bhansali Anil
Department of Hepatology, Postgraduate Institute of Medical Education and Research, Sector 12, Chandigarh 160012, India.
Department of Histopathology, Postgraduate Institute of Medical Education and Research, Sector 12, Chandigarh 160012, India.
J Clin Exp Hepatol. 2013 Mar;3(1):12-8. doi: 10.1016/j.jceh.2012.10.009. Epub 2012 Nov 2.
Oxidative stress and cytokines play an important role in the pathogenesis of nonalcoholic fatty liver disease (NAFLD). We compared the presence of oxidative stress and cytokines in 25 patients with NAFLD with 25 age, sex and BMI-matched patients with chronic viral hepatitis (CVH) and 25 healthy volunteers (HV).
Oxidative stress was studied biochemically by markers of lipid peroxidation and biochemical assessment of anti-oxidant status and various cytokines were studied by ELISA.
Patients with NAFLD had significantly higher levels of malondialdehyde (MDA) (p = 0.000) and conjugated dienes (CD) (p = 0.000) in comparison to HVs. Patients with NAFLD also had significantly higher MDA levels (p = 0.000) in comparison to CVH patients. Patients with NAFLD had significantly lower GSH levels (p = 0.004) in comparison to HVs. Patients with NAFLD had higher GPx activity (p = 0.028) in comparison to HVs. Catalase activity was significantly decreased in both NAFLD (p = 0.001) and CVH patients (p = 0.000) in comparison to HVs. Patients with NAFLD had significantly higher SOD activity (p = 0.000) in comparison to CVH patients. There was no difference in serum levels of IL-1β and TNF-α amongst three groups. Patients with CVH were found to have higher IL-8 serum levels (p = 0.039) in comparison to HVs. CVH patients also had higher TGF-β levels (p = 0.002) in comparison to both NAFLD patients and HVs.
Differences in the markers of oxidative stress and anti-oxidant status between NAFLD, CVH and healthy volunteers suggest presence of higher oxidative stress in patients with NAFLD.
氧化应激和细胞因子在非酒精性脂肪性肝病（NAFLD）的发病机制中起重要作用。我们比较了25例NAFLD患者与25例年龄、性别和体重指数（BMI）匹配的慢性病毒性肝炎（CVH）患者以及25名健康志愿者（HV）体内氧化应激和细胞因子的情况。
通过脂质过氧化标志物进行氧化应激的生化研究，并对抗氧化状态进行生化评估，通过酶联免疫吸附测定法（ELISA）研究各种细胞因子。
与健康志愿者相比，NAFLD患者的丙二醛（MDA）水平（p = 0.000）和共轭二烯（CD）水平（p = 0.000）显著更高。与CVH患者相比，NAFLD患者的MDA水平也显著更高（p = 0.000）。与健康志愿者相比，NAFLD患者的谷胱甘肽（GSH）水平显著更低（p = 0.004）。与健康志愿者相比，NAFLD患者的谷胱甘肽过氧化物酶（GPx）活性更高（p = 0.028）。与健康志愿者相比，NAFLD患者（p = 0.001）和CVH患者（p = 0.000）的过氧化氢酶活性均显著降低。与CVH患者相比，NAFLD患者的超氧化物歧化酶（SOD）活性显著更高（p = 0.000）。三组之间白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的血清水平没有差异。与健康志愿者相比，CVH患者的IL-8血清水平更高（p = 0.039）。与NAFLD患者和健康志愿者相比，CVH患者的转化生长因子-β（TGF-β）水平也更高（p = 0.002）。
NAFLD、CVH和健康志愿者之间氧化应激和抗氧化状态标志物的差异表明，NAFLD患者存在更高的氧化应激。
