Qian Jun, Chen Qin, Yao Dong-Ming, Yang Lei, Yang Jing, Wen Xiang-Mei, Zhang Ying-Ying, Chai Hai-Yan, Ma Ji-Chun, Deng Zhao-Qun, Lin Jiang
Department of Hematology, Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
Laboratory Center, Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
Int J Clin Exp Pathol. 2015 Jan 1;8(1):127-36. eCollection 2015.
Overexpression of MAPK/MAK/MRK overlapping kinase (MOK) has been found in various tumors. However, the mechanism underlying MOK upregulation remains unclear. A CpG island was identified in MOK promoter. In this study, we evaluated the expression and methylation status of MOK gene in acute myeloid leukemia (AML). Hypomethylation of MOK promoter was detected in 31.0% (45/145) of AML patients. The degree of MOK hypomethylation was significantly correlated with MOK expression in AML patients. MOK-hypomethylated patients had a trend towards lower WBCs. Receiver operating characteristic curve (ROC) analysis showed a good performance in distinguishing AML patients from controls with an area under the ROC curve (AUC) of 0.820 (P < 0.001). In summary, our results suggest MOK promoter hypomethylation is a common event and contributes to MOK overexpression in AML.
在多种肿瘤中均发现丝裂原活化蛋白激酶/微管相关蛋白激酶/丝裂原活化蛋白激酶重叠激酶（MOK）过表达。然而，MOK上调的潜在机制仍不清楚。在MOK启动子中鉴定出一个CpG岛。在本研究中，我们评估了急性髓系白血病（AML）中MOK基因的表达和甲基化状态。在31.0%（45/145）的AML患者中检测到MOK启动子低甲基化。MOK低甲基化程度与AML患者中MOK表达显著相关。MOK低甲基化患者的白细胞计数有降低趋势。受试者工作特征曲线（ROC）分析显示，在区分AML患者与对照组方面表现良好，ROC曲线下面积（AUC）为0.820（P < 0.001）。总之，我们的结果表明MOK启动子低甲基化是AML中的常见事件，并导致MOK过表达。
