微小RNA-1179通过SLIT2/ROBO1轴促进食管鳞状细胞癌的细胞侵袭。
miR-1179 promotes cell invasion through SLIT2/ROBO1 axis in esophageal squamous cell carcinoma.
作者信息
Jiang Lixin, Wang Yongfang, Rong Yaxiong, Xu Lianhong, Chu Ying, Zhang Ying, Yao Yonghua
机构信息
Department of Clinical Laboratory, Wujin Hospital Affiliated to Jiangsu University Changzhou, China.
Department of General Surgery, Wujin Hospital Affiliated to Jiangsu University Changzhou, China.
出版信息
Int J Clin Exp Pathol. 2015 Jan 1;8(1):319-27. eCollection 2015.
Abstract
MiR-1179, a new identified miRNA highly associated with metastasis of colorectal cancer which was never reported in esophageal squamous cell carcinoma (ESCC). Here we measured the expression levels of miR-1179 and the candidate target gene in tissues from 40 patients with ESCC. Transwell, Dual-luciferase reporter assay and immunocytochemistry assay were employed to detect the function role of miR-1179 in vitro. We found that miR-1179 was up-regulated in human ESCC tumor tissues. Bioinformatics analysis indicated that SLIT2 acting as a new potential target of miR-1179 which was confirmed by luciferase reporter assay. Down-regulation of miR-1179 suppressed cell invasion in vitro with an increasing level of SLIT2 and ROBO1, besides, the up-regulation of SLIT2 decreased cell invasion through ROBO1. Taken together, these findings will shed light the role to mechanism of miR-1179 in regulating cell invasion via SLIT2/ROBO1 axis.
摘要
MiR-1179是一种新发现的与结直肠癌转移高度相关的微小RNA，在食管鳞状细胞癌（ESCC）中从未有过报道。在此，我们检测了40例ESCC患者组织中miR-1179及其候选靶基因的表达水平。采用Transwell实验、双荧光素酶报告基因检测和免疫细胞化学检测来体外检测miR-1179的功能作用。我们发现miR-1179在人ESCC肿瘤组织中上调。生物信息学分析表明，SLIT2作为miR-1179的一个新的潜在靶点，这一结果通过荧光素酶报告基因检测得到证实。miR-1179的下调抑制了体外细胞侵袭，同时SLIT2和ROBO1水平升高，此外，SLIT2的上调通过ROBO1降低了细胞侵袭。综上所述，这些发现将揭示miR-1179通过SLIT2/ROBO1轴调节细胞侵袭的作用机制。
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