Chu Xia, Zhao Po, Lv Yali, Liu Lin
Department of Pathology, 153 Hospital of PLA Zhengzhou 450042, China ; Department of Pathology, PLA General Hospital 28 Fuxing Road, Beijing 100853, China.
Department of Pathology, PLA General Hospital 28 Fuxing Road, Beijing 100853, China.
Int J Clin Exp Pathol. 2015 Jan 1;8(1):328-36. eCollection 2015.
Recent findings suggest decreasing TFPI-2 expression plays a significant role in inhibiting cell migration and tumor invasion. The clinicopathological significance of the expression of TFPI-2 and its possible correlation with the expression of CD133 in cholangiocarcinoma remains to be solved.
We investigated if TFPI-2 was involved in the clinicopathological significance of cholangiocarcinoma. An immunohistochemical method was used to detect 218 cases of cholangiocarcinoma, 30 para-neoplastic and 20 normal bile ducts for their expression status of TFPI-2 and CD133, and then the results were analyzed with the patient's age, sex, tumor site and the histological grade, clinical stage as well as overall mean survival time.
Compared with the para-neoplastic and normal cholangiocytes, the expression of TFPI-2 was obviously decreased while the expression of CD133 in carcinoma cells was increased. Carcinomas with low expression of TFPI-2 were significantly corresponding to the tumor site (P = 0.006), size (P = 0.005), histological grade (P = 0.0001) and clinical stage (P = 0.0001), but not to the age (P = 0.066) and sex (P = 0.411), respectively. By Kaplan-Meier survival analysis, the low expression of TFPI-2 was significantly correlative with the overall survival time (P = 0.0001). Further, the expression of TFPI-2 was found inversely correlative with the expression of CD133 (g = -0.3876, P < 0.0001).
Our finding suggests that the decreased expression of TFPI-2 may play an important role in the carcinogenesis and progression, and may become a new adjunct marker in the diagnosis and prognosis in cholangiocarcinoma. The expression of TFPI-2 may be inversely correlative with the expression of CD133.
最近的研究结果表明，组织因子途径抑制物-2（TFPI-2）表达降低在抑制细胞迁移和肿瘤侵袭中起重要作用。TFPI-2表达的临床病理意义及其与胆管癌中CD133表达的可能相关性仍有待解决。
我们研究了TFPI-2是否与胆管癌的临床病理意义有关。采用免疫组织化学方法检测218例胆管癌、30例癌旁组织及20例正常胆管中TFPI-2和CD133的表达情况，然后将结果与患者的年龄、性别、肿瘤部位、组织学分级、临床分期以及总平均生存时间进行分析。
与癌旁及正常胆管细胞相比，癌细胞中TFPI-2的表达明显降低，而CD133的表达增加。TFPI-2低表达的癌分别与肿瘤部位（P = 0.006）、大小（P = 0.005）、组织学分级（P = 0.0001）和临床分期（P = 0.0001）显著相关，但与年龄（P = 0.066）和性别（P = 0.411）无关。通过Kaplan-Meier生存分析，TFPI-2低表达与总生存时间显著相关（P = 0.0001）。此外，发现TFPI-2的表达与CD133的表达呈负相关（r = -0.3876，P < 0.0001）。
我们的研究结果表明，TFPI-2表达降低可能在胆管癌的发生和发展中起重要作用，并可能成为胆管癌诊断和预后的新辅助标志物。TFPI-2的表达可能与CD133的表达呈负相关。
