Arakawa Takahiro, Iitani Kenta, Wang Xin, Kajiro Takumi, Toma Koji, Yano Kazuyoshi, Mitsubayashi Kohji
Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Analyst. 2015 Apr 21;140(8):2881-6. doi: 10.1039/c4an02390k. Epub 2015 Mar 10.
A two-dimensional imaging system (Sniffer-camera) for visualizing the concentration distribution of ethanol vapor emitting from wine in a wine glass has been developed. This system provides image information of ethanol vapor concentration using chemiluminescence (CL) from an enzyme-immobilized mesh. This system measures ethanol vapor concentration as CL intensities from luminol reactions induced by alcohol oxidase and a horseradish peroxidase (HRP)-luminol-hydrogen peroxide system. Conversion of ethanol distribution and concentration to two-dimensional CL was conducted using an enzyme-immobilized mesh containing an alcohol oxidase, horseradish peroxidase, and luminol solution. The temporal changes in CL were detected using an electron multiplier (EM)-CCD camera and analyzed. We selected three types of glasses-a wine glass, a cocktail glass, and a straight glass-to determine the differences in ethanol emission caused by the shape effects of the glass. The emission measurements of ethanol vapor from wine in each glass were successfully visualized, with pixel intensity reflecting ethanol concentration. Of note, a characteristic ring shape attributed to high alcohol concentration appeared near the rim of the wine glass containing 13 °C wine. Thus, the alcohol concentration in the center of the wine glass was comparatively lower. The Sniffer-camera was demonstrated to be sufficiently useful for non-destructive ethanol measurement for the assessment of food characteristics.
已开发出一种二维成像系统（嗅探相机），用于可视化从酒杯中的葡萄酒散发出来的乙醇蒸汽的浓度分布。该系统利用固定有酶的网产生的化学发光（CL）来提供乙醇蒸汽浓度的图像信息。该系统通过测量酒精氧化酶和辣根过氧化物酶（HRP）-鲁米诺-过氧化氢体系引发的鲁米诺反应产生的CL强度来测定乙醇蒸汽浓度。使用含有酒精氧化酶、辣根过氧化物酶和鲁米诺溶液的固定有酶的网，将乙醇分布和浓度转换为二维CL。使用电子倍增器（EM）-CCD相机检测并分析CL的时间变化。我们选择了三种类型的杯子——一个葡萄酒杯、一个鸡尾酒杯和一个直筒杯——来确定杯子形状效应导致的乙醇散发差异。成功地可视化了每个杯子中葡萄酒乙醇蒸汽的散发测量结果，像素强度反映了乙醇浓度。值得注意的是，在装有13°C葡萄酒的葡萄酒杯边缘附近出现了一个归因于高酒精浓度的特征性环形。因此，葡萄酒杯中心的酒精浓度相对较低。结果表明，嗅探相机对于无损乙醇测量以评估食品特性非常有用。
