Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Japan.
Luminescence. 2012 Sep-Oct;27(5):328-33. doi: 10.1002/bio.1352. Epub 2011 Oct 5.
A two-dimensional gaseous ethanol visualization system has been developed and demonstrated using a horseradish peroxidase-luminol-hydrogen peroxide system with high-purity luminol solution and a chemiluminescence (CL) enhancer. This system measures ethanol concentrations as intensities of CL via the luminol reaction. CL was emitted when the gaseous ethanol was injected onto an enzyme-immobilized membrane, which was employed as a screen for two-dimensional gas visualization. The average intensity of CL on the substrate was linearly related to the concentration of standard ethanol gas. These results were compared with the CL intensity of the CCD camera recording image in the visualization system. This system is available for gas components not only for spatial but also for temporal analysis in real time. A high-purity sodium salt HG solution (L-HG) instead of standard luminol solution and an enhancer, eosin Y (EY) solution, were adapted for improvement of CL intensity of the system. The visualization of gaseous ethanol was achieved at a detection limit of 3 ppm at optimized concentrations of L-HG solution and EY.
已开发并展示了一种二维气态乙醇可视化系统，该系统使用辣根过氧化物酶-鲁米诺-过氧化氢体系，采用高纯度鲁米诺溶液和化学发光（CL）增强剂。该系统通过鲁米诺反应将乙醇浓度测量为 CL 的强度。当气态乙醇注入到酶固定化膜上时，会发出 CL，该膜用作二维气体可视化的屏幕。基底上 CL 的平均强度与标准乙醇气体的浓度呈线性关系。这些结果与可视化系统中 CCD 相机记录图像的 CL 强度进行了比较。该系统不仅可用于空间气体成分的实时分析，还可用于时间分析。高纯度 HG 钠盐溶液（L-HG）代替标准鲁米诺溶液和增强剂曙红 Y（EY）溶液，以提高系统的 CL 强度。在优化的 L-HG 溶液和 EY 浓度下，实现了对气态乙醇的可视化，检测限为 3ppm。
