Callard G, Mak P, DuBois W, Cuevas M E
Department of Biology, Boston University, Massachusetts 02215.
J Exp Zool Suppl. 1989;2:23-34. doi: 10.1002/jez.1402520405.
Spermatogenesis is a unique developmental sequence dependent on FSH and androgen. Due to the complex organization of the mammalian testis, however, mechanistic details of regulation are largely unknown. Using the dogfish shark (Squalus acanthias) in which there is a cystic mode of spermatogenesis and a topographic separation of different germ cell stages within the testis, we have obtained new information of general relevance on stage-related biochemical and morphological changes and have proposed a model in which steroids serve as parahormonal regulators of the spermatogenic progression. In addition, techniques developed for culturing staged spermatocysts (intact Sertoli/germ cell units) and isolated, staged Sertoli cells demonstrate the usefulness of this model for studying spermatogenic regulation under defined conditions in vitro.
精子发生是一种依赖促卵泡激素（FSH）和雄激素的独特发育过程。然而，由于哺乳动物睾丸组织复杂，调控的机制细节在很大程度上尚不清楚。通过使用白斑角鲨（Squalus acanthias），其精子发生具有囊性模式且睾丸内不同生殖细胞阶段存在拓扑分离，我们获得了与阶段相关的生化和形态学变化的新的普遍相关信息，并提出了一个模型，其中类固醇作为精子发生进程的旁分泌激素调节剂。此外，用于培养分期精母细胞囊肿（完整的支持细胞/生殖细胞单位）和分离的分期支持细胞的技术证明了该模型在体外特定条件下研究精子发生调控的有用性。
