Camargo Anderson Carlos, de Castilho Natalia Parma Augusto, da Silva Danilo Augusto Lopes, Vallim Deyse Christina, Hofer Ernesto, Nero Luís Augusto
1 Departamento de Veterinária, Universidade Federal de Viçosa , Viçosa, Minas Gerais, Brazil .
2 Laboratório de Zoonoses Bacterianas, Instituto Oswaldo Cruz , Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil .
Microb Drug Resist. 2015 Aug;21(4):458-62. doi: 10.1089/mdr.2014.0270. Epub 2015 Mar 10.
The present study aimed to assess the antimicrobial resistance and the presence of virulence markers in 137 Listeria monocytogenes isolates obtained from meat-processing environments, beef products, and clinical cases. All isolates were subject to molecular serogrouping and their antibiotic resistance profiles were assessed against 12 antimicrobials. In addition, isolates were subjected to detection of virulence marker genes (inlA, inlC, inlJ). The isolates were classified into serogroups 4b, 4d, 4a, or 4c (46%), 1/2c or 3c (27%), 1/2a or 3a (13.9%), and 1/2b or 3b (13.1%). All tested isolates presented sensitivity to the majority of the tested antimicrobials, but most of them presented resistance or intermediate resistance to clindamycin (88.3%) and oxacillin (73.7%). Virulence markers were detected in all isolates, demanding further analysis to better characterize their pathogenic potential.
本研究旨在评估从肉类加工环境、牛肉产品和临床病例中分离出的137株单核细胞增生李斯特菌的抗菌耐药性和毒力标记物的存在情况。所有分离株均进行了分子血清分型，并评估了它们对12种抗菌药物的耐药谱。此外，对分离株进行了毒力标记基因（inlA、inlC、inlJ）的检测。分离株被分为血清型4b、4d、4a或4c（46%）、1/2c或3c（27%）、1/2a或3a（13.9%）以及1/2b或3b（13.1%）。所有测试的分离株对大多数测试抗菌药物敏感，但其中大多数对克林霉素（88.3%）和苯唑西林（73.7%）呈现耐药或中介耐药。在所有分离株中均检测到毒力标记物，需要进一步分析以更好地表征其致病潜力。
