Eanes W F, Ajioka J W, Hey J, Wesley C
Department of Ecology and Evolution, State University of New York, Stony Brook 11794.
Mol Biol Evol. 1989 Jul;6(4):384-97. doi: 10.1093/oxfordjournals.molbev.a040555.
Restriction-map variation was studied in 126 copies of the G6pd region in X chromosome lines of Drosophila melanogaster from North America, Europe, and Africa. Special attention was focused on the distribution of variation relative to the geographically variable polymorphism for two electrophoretic variants. Nucleotide heterozygosity as determined by eight six-cutter restriction enzymes for the 13-kb region is estimated, on the basis of the worldwide sample, to be 0.065%, which is the lowest value reported for any comparable region in the D. melanogaster genome. Significant linkage disequilibrium between electrophoretic alleles and restriction-site variation is observed for several sites. In contrast to published studies of other genetic regions, there are large insertions that reach significant frequencies and are found across considerable geographic distances. There is a clustering of this variation inside the first large intervening sequence of the G6PD gene.
对来自北美、欧洲和非洲的黑腹果蝇X染色体品系中126份G6pd区域进行了限制性图谱变异研究。特别关注了相对于两种电泳变体的地理可变多态性的变异分布。基于全球样本估计，由八种六切点限制性内切酶测定的13 kb区域的核苷酸杂合度为0.065%，这是黑腹果蝇基因组中任何可比区域报道的最低值。在几个位点观察到电泳等位基因与限制性位点变异之间存在显著的连锁不平衡。与其他遗传区域的已发表研究相反，存在达到显著频率且在相当大地理距离上发现的大插入片段。这种变异在G6PD基因的第一个大间隔序列内聚集。
